Skip to main content

Advertisement

Effects of denosumab on bone mineral density and bone turnover markers in rheumatoid arthritis patients switching from bisphosphonates

Article metrics

  • 261 Accesses

Abstract

Background

To compare the efficacy of 12-month denosumab treatment on bone mineral density (BMD) and bone turnover markers (BTMs) between treatment-naïve osteoporosis patients with rheumatoid arthritis (RA) and those with previous bisphosphonate (BP) therapy.

Methods

A total of 36 RA patients with osteoporosis completed 12-month follow-up. Twenty-five patients were osteoporotic treatment-naïve (naïve group), and 11 patients were previously treated with BPs (switch group) (average 7.9 years). BMD and BTMs were measured before and 6 and 12 months after treatment.

Results

BTM levels were higher in the naïve group at baseline. However, the same level of suppression was achieved at 6 months in both groups. Spine BMD increased significantly in both groups. There was no significant difference in the mean percent changes of BMD of the spine (naïve group: 6.8 ± 0.8, switch group: 5.1 ± 1.5), femoral neck (2.9 ± 1.4, 2.9 ± 1.3), and total hip (1.7 ± 0.9, 1.4 ± 1.1) between these two groups at 12 months.

Conclusions

The effects of denosumab on BMD and BTMs of the switch group after long-term BP treatment are comparable to those of the naïve group in RA patients. Thus, switching BPs to denosumab is one of the useful options to treat osteoporosis with RA.

Background

Rheumatoid arthritis (RA) is an inflammatory disease in which systemic arthritis causes significant functional impairment. Generalized bone loss leading to osteoporosis is one of the extra-articular manifestations of RA (Vis et al. 2006) and may lead to the occurrence of fragility fractures, which further increase the burden of the disease. Therefore, RA patients often require treatment for osteoporosis in addition to RA treatment. RA-related risk factors for osteoporosis, such as body mass index (BMI), disease duration and activity, level of disability, corticosteroid and disease-modifying anti-rheumatic drug (DMARD) usage, menopausal status, and production of pro-inflammatory cytokines, have been classified and studied (Gough et al. 1994; Haugeberg et al. 2000; Laan et al. 1992; Sinigaglia et al. 2000). It is known that upregulation of pro-inflammatory cytokines such as tumor necrosis factor (TNF)-α, interleukin (IL)-1, and IL-6 leads to bone resorption through overexpression of receptor activator of nuclear factor kappa-B ligand (RANKL) and activated T cells (Kotake et al. 2001).

Anti-resorptive agents, such as bisphosphonates (BPs), have a high affinity for bone minerals and inhibit bone resorption by osteoclasts (Reszka and Rodan 2003). BPs have high-level evidence for fracture risk reduction in postmenopausal osteoporotic women (Wells et al. 2008a; Wells et al. 2008b). Some reports have shown that several BPs (alendronate, risedronate, zoledronate) have positive effects on bone mineral density (BMD) in patients with inflammatory rheumatic conditions (Benucci et al. 2009; den Uyl et al. 2011; Hakala et al. 2012). Therefore, BPs have mainly been used for the prevention of fragility fractures in RA patients. However, there is no evidence for how long osteoporotic patients should be treated with BPs, and which agent should be administered to RA patients with osteoporosis after the treatment with BPs.

Another anti-resorptive agent, denosumab, is an antibody against RANKL, which is essential for osteoclast differentiation, activation, and survival (Fuller et al. 1998; Lacey et al. 2000; Lacey et al. 1998; Yasuda et al. 1998). Denosumab potently reduces bone resorption through binding RANKL, leading to increases in BMD and cortical bone microstructure in postmenopausal patients with osteoporosis (Genant et al. 2010; Poole et al. 2015). Moreover, previous reports of denosumab have shown not only increases in lumbar spine and hip BMD, but also inhibition of progression of bone erosion in RA patients (Cohen et al. 2008; Mok et al. 2015; Rossini et al. 2016; Takeuchi et al. 2016). These observations suggest that denosumab has positive effects on not only the prevention of fragility fractures, but also on joint damage in RA patients.

Recently, Ebina et al. reported that the switching BPs to denosumab showed a significant increase in BMD compared to those with continued BPs in RA patients, and concluded that switching BPs to denosumab in RA might provide some useful osteoporosis treatment options (Ebina et al. 2018). Therefore, it should be evaluated whether the clinical efficacy of denosumab following BPs could be comparable to those with treatment-naïve patients with osteoporosis in RA.

The purpose of this study was to compare the effects of denosumab on BMD and bone remodeling markers between treatment-naïve patients and patients previously treated with long-term BPs.

Materials and methods

Patients

This study was an observational study in the Gunma Rheumatoid Arthritis Network, and the ethics committee of Gunma University approved the protocol (IRB approval No 23–37). Informed consent was obtained from all individual participants included in the study. The subjects were 42 women RA patients aged 50 years and older who started receiving denosumab treatment from May 2014 to June 2015 at our hospital. All patients fulfilled the American College of Rheumatology (ACR) classification criteria (1987) (Arnett et al. 1988) and they had received anti-rheumatic drugs (glucocorticoids (PSL), salazosulfapyridine, iguratimod, bucillamine, tacrolimus, methotrexate (MTX), bailogical disease-modifying anti-rheumatic drugs (bDMARDs)) prior to this study. The patients were diagnosed with concurrent osteoporosis, defined as 1) femoral neck or vertebral fragility fractures or 2) young adult mean BMD less than 70% without a fragility fracture. Patients with other inflammatory diseases, class 4 Steinbrocker classification (Steinbrocker et al. 1949), serious renal or hepatic dysfunction, or previous spinal or hip procedures were excluded. Twenty-eight of 42 patients were treatment-naïve osteoporotic RA patients. The effects of denosumab administered to RA patients with no previous treatment (naïve group) were compared to those with BP pre-treatment (switch group). Denosumab (60 mg, Daiichi-Sankyo, Tokyo, Japan) was injected subcutaneously every 6 months. All patients received supplements of calcium 610 mg, magnesium 30 mg and vitamin D 400 IU per day throughout the study. The mean duration of prior BP treatment was 7.9 years in the switch group. The BPs that had been used were alendronate (n = 3) and risedronate (n = 8).

Clinical assessment

The serum levels of total-procollagen type I N-terminal propeptide (total-PINP), tartrate-resistant acid phosphatase-5b (TRACP-5b), and undercarboxylated osteocalcin (ucOC), and the BMDs of the lumbar spine (LS) (L1-L4), the left femoral neck (FN), and the left total hip (TH) on dual-energy X-ray absorptiometry (DCS-900EX; Hitachi, Tokyo, Japan) were measured before and 6 and 12 months after the start of treatment. The disease activity scores of 28 joints based on the erythrocyte sedimentation rate (DAS28-ESR) and the simplified disease activity index (SDAI) were used to evaluate RA disease activity (Aletaha and Smolen 2005). Use of methotrexate (MTX), glucocorticoids (PSL), biological disease-modifying anti-rheumatic drugs (bDMARDs), and other conventional synthetic disease-modifying anti-rheumatic drugs (csDMARDs) was also investigated.

Statistical analysis

Statistical analysis was conducted using SPSS version 22 (IBM Inc., Chicago, IL, USA). Results are reported as means ± standard deviation (SD). A p value less than 0.05 was considered significant. Chi-squared tests or Fisher’s exact tests were used for comparisons between two groups for categorical variables, and Mann-Whitney U tests were used to assess continuous variables. Wilcoxon’s signed-rank sum test or McNemar’s test was used to assess differences between the groups, as appropriate.

Results

A total of 36 patients completed 12-month follow-up. A total of 6 patients discontinued the study, 3 in the naïve group and 3 in the switch group; of these patients, 2 in the naïve group and 3 in the switch group discontinued because they were lost to follow-up, and 1 patient in the naïve group discontinued because of joint surgery (Fig. 1).

Fig. 1
figure1

Trial profile. RA: rheumatoid arthritis; Naive group: treatment-naïve group; Switch group: transitioning from bisphosphonate group; BPs: bisphosphonates

The clinical characteristics of the patients are shown in Table 1. There were no significant differences in height, weight, and BMD between the groups. However, mean age and percentage with previous fragility fractures were significantly higher in the switch group than in the naïve group. Furthermore, baseline total-P1NP, TRACP-5b, and ucOC levels were significantly higher in the naïve group than in the switch group.

Table 1 Baseline characteristics

RA disease duration, disease activity, and medical condition at baseline were not significantly different between the 2 groups (Table 2).

Table 2 Baseline characteristics of RA

Table 3 shows the changes in DAS28-ESR, SDAI, and the use of MTX, PSL, bDMARDs, and csDMARDs during this study. These parameters also showed no significant differences from baseline to 6 and 12 months after treatment initiation.

Table 3 Changes of disease activities and medications

Table 4 shows the changes of serum levels of total-PINP, TRACP-5b, and ucOC and of BMD. In the naïve group, there were significant decreases in all BTMs at 6 months and 12 months from baseline. On the other hand, in the switch group, there were no significant decreases in all BTMs at 6 and 12 months compared to baseline except for ucOC at 6 months. At 6 and 12 months, the BTM levels were not significantly different between the two groups, although baseline levels were higher in the naïve group than in the switch group (Table 5). Spine BMD was significantly increased in both groups from baseline to 6 and 12 months. Femoral neck (FN) BMDs at 6 months in both groups and at 12 months in the naïve group were significantly increased from baseline. However, in the switch group, there was no significant increase in FN BMD at 12 months. TH BMD was significantly increased only in the naïve group at 12 months.

Table 4 Changes of bone turnover markers and BMD in the naïve and switch groups after 6 and 12 months of treatment
Table 5 The bone turnover markers levels at 6 and 12 months in the naïve and switch groups

Figure 2 shows the mean percent changes of lumbar spine and hip BMDs from baseline. There were no significant differences between the groups in the mean percent changes of BMD of the spine, FN, and TH at 6 months (naïve group: 4.5 ± 0.5, 3.2 ± 1.1, 1.3 ± 1.1, switch group: 4.2 ± 1.4, 3.0 ± 1.2, 1.6 ± 1.1, respectively) and at 12 months (naïve group: 6.8 ± 0.8, 2.9 ± 1.4, 1.7 ± 0.9, switch group: 5.1 ± 1.5, 2.9 ± 1.3, 1.4 ± 1.1, respectively). No subjects experienced any clinical fractures or serious adverse effects during the course of this study.

Fig. 2
figure2

Mean percent changes from baseline in lumbar spine and hip BMDs. Naive group: treatment-naïve group; switch group: transitioning from bisphosphonate group; BMD: bone mineral density; FN: femoral neck; TH: total hip. P values from Mann-Whitney U tests, for the mean percent change from baseline in the naive group versus the switch group. n.s denotes no significant difference between the values

Discussion

In this study, the effect of denosumab in RA patients previously treated with long-term (average 7.9 years) BPs (switch group) was compared to that in treatment-naïve RA patients (naïve group). At 6 and 12 months, the BTM levels were similar between the two groups, although baseline levels in the naïve group were significantly higher than those in the switch group.

Nakamura et al. reported a direct comparison of BMD changes between the treatment-naïve and the BPs pre-treated patients at the 24 months. They demonstrated that the denosumab had an equal effect on the BMD increase to the treatment-naïve group and the group with BPs treatment over 15 years (Nakamura et al. 2017). Although the duration of pre-treatment with BPs was shorter than their study, this study showed significantly increased spine BMD in both groups from baseline to 6 and 12 months, and the significant increase in FN BMD was observed from baseline to 6 months in both groups. In addition, the mean percent changes in BMD were not significantly different between the two groups.

A similar study of denosumab for postmenopausal patients reported a direct comparison of BMD changes at 12 months in treatment-naïve patients and patients pre-treated with zoledronic acid. Pre-treated postmenopausal patients showed significant increases in lumbar spine and hip BMDs, and these increases did not differ significantly between the two groups (Anastasilakis et al. 2015). Moreover, previous reports have indicated that BPs reduce bone resorption through a different mechanism than denosumab (Lacey et al. 1998; Russell et al. 2008). The present results also suggest that prior BP treatment does not negatively affect subsequent denosumab treatment in RA patients, at least in the short term.

On the other hand, in the switch group, there was no significant increase in FN and TH BMDs from baseline to 12 months. Moreover, the mean percent changes of lumbar BMD were larger in the naïve group than in the switch group, although the difference was not significant (Fig. 2). The previous study also reported that denosumab without pre-treatment had a significantly higher percent change in the hip total BMD at 6 months in RA patients (Nakamura et al. 2017). These might be attributable to the already-decreased bone remodeling due to BP treatment before initiation of denosumab treatment.

In this study, BTM levels were higher in the naïve group at baseline as expected. In the naïve group, they were significantly decreased from baseline at all time periods. In the switch group, they were not significantly decreased, except for ucOC at 6 months. However, although starting from a much different baseline level, BTMs reached similar levels of suppression at 6 months in both groups (Table 5). This finding suggests that previous treatment with BPs, such as alendronate or risedronate, does not attenuate the suppression of bone turnover achieved by denosumab.

Inhibition of RANKL has been shown to prevent inflammatory bone loss in patients with chronic inflammatory arthritis (Schett et al. 2005) and to prevent both bone erosion in the subchondral bone and cartilage destruction (Takeuchi et al. 2016). Since this study demonstrated that short term administration of denosumab had a significant increase in BMD and decrease in the BTM levels, the denosumab could be the first osteoporotic agent for the treatment with osteoporosis in RA patients. On the other hand, in the present study, denosumab did not affect disease activity, including inflammation, in RA patients, similar to the findings of a past report (Kinoshita et al. 2016). From these observations, we consider that when using denosumab to treat RA, it is better to use it in combination with some anti-rheumatic disease agents.

In general, there is a positive relationship between the course of RA and the degree of both local and generalized bone losses, that is, cumulatively increased disease activity is associated with systemic bone loss. Therefore, treatment with DMARDs is of major importance, not only to improve disease activity, but also to prevent generalized bone loss. Several studies have shown that bDMARD treatment, mostly combined with MTX, protected against generalized bone loss and impaired bone quality (Kageyama et al. 2008; Marotte et al. 2007; Wijbrandts et al. 2009). Moreover, the use of glucocorticoids increases the risk of fragility fracture due to inhibition of the production, survival, and differentiation of osteoblasts (Chua et al. 2003; Compston 2010; Emkey and Epstein 2014) and enhancement of the survival and activity of osteoclasts by increasing RANKL expression (Sivagurunathan et al. 2005). In the present study, the percentage using PSL was higher in the switch group than in the naïve group. This background difference between the groups may have had a slight effect on the results.

All subjects of this study had received some kind of DMARD treatment for RA, and the percentage of patients using bDMARDs was 32.0% in the naïve group and 54.5% in the switch group. Although there was slightly higher, but not significantly higher, use of bDMARDs in the switch group, use of bDMARDs was considered to have little effect on the changes in BMD and BTMs, because there was no change of bDMARDs during the 12 months (Table 3). Moreover, previous reports demonstrated that the use of bDMARDs and disease control by bDMARDs had not increased, but decreases in the BMD of the hip, spine, and/or hand were prevented. (Bertoldi et al. 2013; Marotte et al. 2007; Wijbrandts et al. 2009).

It has been reported that oral corticosteroid treatment with more than 5 mg daily of prednisolone or equivalent reduces bone mineral density and causes a rapid increase in the risk of fracture during the treatment period (Van Staa et al. 2002). Previous denosumab studies showed that the increases of BMD in glucocorticoid users were expected to be lower than those reported in studies of postmenopausal women (Brown et al. 2009; Kendler et al. 2010; Mok et al. 2015; Recknor et al. 2013). In the present study, 54.5% of patients in the switch group had used PSL, with an average dosage of 3.2 mg/day. This group also had slightly higher disease activities. Thus, although the dose of PSL did not exceed 5 mg/day, it was possible that the combination of PSL and slightly higher disease activity might have affected the increase in BMD in the switch group.

There are several limitations to this study. First, the number of subjects for each parameter at follow-up was small, and comparisons of these data may thus have been affected by number bias, especially the data for age, previous fragility fractures, and the percentages of use of bDMARDs and glucocorticoids as stated above. Although there was no significant difference between the groups in the percentage of use of bDMARDs and PSL on statistical analysis, the switch group had a significantly older age and a higher percentage of fragility fractures; thus, some conditional bias might be expected in comparisons between these groups. Second, this study was not a randomized, controlled trial; thus, selection bias may have been present. Third, the observation period of 12 months was short, and long-term efficacy remains unknown. Fourth, there was no control group. One was the treatment-naïve patients receiving a placebo, and the other was continued BPs group, which allowed to compare the clinical effect of denosumab over BPs. Finally, fracture risk reductions in these patients were not shown in this study. In order to elucidate the above points, results from a long-term study with a large number of patients would be needed.

Conclusions

In conclusion, a direct comparison between the switch group from long-term BPs treatment and the treatment-naïve group in RA patients demonstrated that there were no significant differences between the groups in the mean percent changes of BMDs of the lumbar spine, FN, and TH at 6 months and 12 months. These data indicated that denosumab’s effectiveness on BMD in the switch group might be comparable to those in the naïve group. Thus, switching BPs to denosumab might be a useful option to treat osteoporosis with RA.

Availability of data and materials

The data that support the findings of this study are available from the corresponding author upon reasonable request.

Abbreviations

ACR:

American College of Rheumatology

bDMARDs:

biological disease-modifying anti-rheumatic drugs

BMD:

bone mineral density

BMI:

body mass index

BP:

bisphosphonate

BTM:

bone turnover marker

csDMARDs:

conventional synthetic disease-modifying anti-rheumatic drugs

DAS28-ESR:

disease activity scores of 28 joints based on the erythrocyte sedimentation rate

DMARD:

disease-modifying anti-rheumatic drug

FN:

femoral neck

IL:

interleukin

MTX:

methotrexate

PINP:

procollagen type I N-terminal propeptide

PSL:

methotrexate

RA:

rheumatoid arthritis

RANKL:

receptor activator of nuclear factor kappa-B ligand

SD:

standard deviation

SDAI:

simplified disease activity index

TH:

total hip

TNF:

tumor necrosis factor

TRACP-5b:

tartrate-resistant acid phosphatase-5b

ucOC:

undercarboxylated osteocalcin

References

  1. Aletaha D, Smolen J (2005) The simplified disease activity index (SDAI) and the clinical disease activity index (CDAI): a review of their usefulness and validity in rheumatoid arthritis. 23(5):S100

  2. Anastasilakis AD, Polyzos SA, Efstathiadou ZA, Savvidis M, Sakellariou GT, Papatheodorou A et al (2015) Denosumab in treatment-naïve and pre-treated with zoledronic acid postmenopausal women with low bone mass: effect on bone mineral density and bone turnover markers. Metabolism. 64(10):1291–1297

  3. Arnett FC, Edworthy SM, Bloch DA, Mcshane DJ, Fries JF, Cooper NS et al (1988) The American rheumatism association 1987 revised criteria for the classification of rheumatid arthritis. Arthritis Rheum 31(3):315–324

  4. Benucci M, Saviola G, Baiardi P, Abdi-Ali L, Povino MR, Dolenti S et al (2009) Effects of monthly intramuscular neridronate in rheumatic patients in chronic treatment with low-dose glucocorticoids. Clin Exp Rheumatol 27:567–573

  5. Bertoldi L, Filippou G, Scirè CA, Picemo V, di Sabatino V, Pierquidi S, Galeazzi M, Frediani B (2013) Disease activity and bone density of mcp joints in patients with rheumatoid and psoriatic arthritis: is there a correlation? –a study in patients treated with methotrexate and an anti-TNF α agent. ISRN Rheumatol 2013:708323

  6. Brown JP, Prince RL, Deal C, Recker RR, Kiel DP, de Gregorio LH et al (2009) Comparison of the effect of denosumab and alendronate on BMD and biochemical markers of bone mass: a randomized, blinded, phase 3 trial. J Bone Miner Res 24:153–161

  7. Chua CC, Chua BH, Chen Z, Landy C, Hamdy RC (2003) Dxamethasone induces caspase activation in murine osteoblastic MC3T3-E1 cells. Biochim Biophys Acta 2003; 1642: 79–85

  8. Cohen SB, Dore RK, Lane NE, Ory PA, Peterfy CG, Sharp JT et al (2008) Denosumab treatment effects on structural damage, bone mineral density, and bone turnover in rheumatoid arthritis: a twelve-month, multicenter, randomized, double-blined, pracebo-controlled, phase II clinical trial. Arthritis Rheum 58(5):1299–1309

  9. Compston J (2010) Management of glucocorticoid-induced osteoporosis. Nat Rev Rheumatol 6(2):82–88

  10. den Uyl D, Bultink IE, Lems WF (2011) Glucocorticoid-induced osteoporosis. Clin Exp Rheumatol 29(5):S93–S98

  11. Ebina K, Hirao M, Hashimoto J, Hagihara K, Kashii M, Kitaguchi K et al (2018) Assesment of the effects of switching oral bisphosphonates to denosumab or daily teriparatide in patients with rheumatoid arthritis. J Bone Miner Metab 36(4):478–487

  12. Emkey GR, Epstein S (2014) Secondary osteoporosis: pathophysiology and diagnosis. Best Pract Res Clin Endocrinol Metab 28(6):911–935

  13. Fuller K, Wong B, Fox S, Choi Y, Chambers TJ (1998) TRANCE is necessary and sufficient for osteoblast-mediated activation of bone resorption in osteoclasts. J Exp Med 188(5):997–1001

  14. Genant HK, Engelke K, Hanley DA, Brown JP, Omizo M, Bone HG et al (2010) Denosumab improves density and strength parameters as measured by QCT of the radius in postmenopausal women with low bone mineral density. Bone. 47(1):131–139

  15. Gough AK, Peel NF, Eastell R, Holder RL, Lilley J, Emery P (1994) Excretion of pyridinium crosslinks correlates with disease activity and appendicular bone loss in early rheumatoid arthritis. Ann Rheum Dis 53(1):14–17

  16. Hakala M, Kröger H, Valleala H, Hienonen-Kempas T, Lehtonen-Veromaa M, Heikkinen J et al (2012) Once-monthly oral ibandronate provides significant improvement in bone mineral density in postmenopausal women treated with glucocorticoids for inflammatory rheumatic disease: a 12-month, randomized, double-blind, placebo-controlled trial. Scand J Rheumatol 41(4):260–266

  17. Haugeberg G, Uhlig T, Falch JA, Halse JI, Kvien TK (2000) Bone mineral density and frequency of osteoporosis in female patients with rheumatoid arthritis: result from 394 patients in the Oslo County rheumatoid arthritis register. Arthritis Rheum 43(3):522–530

  18. Kageyama Y, Takahashi M, Nagafusa T, Torikai E, Nagano A (2008) Etanercept reduces the oxidative stress marker levels in patients with rheumatoid arthritis. Reumatol Int 28(3):245–251

  19. Kendler DL, Roux C, Benhamou CL, Brown JP, Lillestol M, Siddhanti S et al (2010) Effects of denosumab on bone mineral density and bone turnover in postmenopausal women transitioning from alendronate therapy. J Bone Miner Res 25(1):72–81

  20. Kinoshita H, Miyakoshi N, Kashiwagura T, Kasukawa Y, Sugimura Y, Shimada Y (2016) Comparison of the efficacy of denosumab and bisphosphonates for treating secondary osteoporosis in patients with rheumatoid arthritis. Mod Rheumatol 27(4):582–586

  21. Kotake S, Udagawa N, Hakoda M, Mogi M, Yano K, Tsuda E et al (2001) Activated human T cells directly induce osteoclastogenesis from human monocytes: possible role of T cells in bone destruction in rheumatoid arthritis patients. Arthritis Rheum 44(5):1003–1012

  22. Laan RFJM, Van Riel PLCM, van Erning LTO, Lemmens JAM, Ruijs SHJ, van de Putte LBA (1992) Vertebral osteoporosis in rheumatoid arthritis patients: effect of low dose prednisone therapy. Rheumatol. 31(2):91–96

  23. Lacey DL, Tan HL, Lu J, Kaufman S, Van G, Qiu W et al (2000) Osteoprotegerin ligand modulates murine osteoclast survival in vitro and in vivo. Am J Pathol 157(2):435–448

  24. Lacey DL, Timms E, Tan H, Kelley MJ, Dunstan CR, Burgess T et al (1998) Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation. Cell. 93(2):165–176

  25. Marotte H, Pallot-Prades B, Grange L, Gaudin P, Alexandre C, Miossec P (2007) A 1-year case-control study in patients with rheumatoid arthritis indicates prevention of loss of bone mineral density in both responders and nonresponders to infliximab. Arthritis Res Ther 9(3):R61

  26. Mok CC, Ho LY, Ma KM (2015) Switching of oral bisphosphonates to denosumab in chronic glucocorticoid users: a 12-month randomized controlled trial. Bone. 75:222–228

  27. Nakamura Y, Suzuki T, Kato H (2017) Denosumab significantly improves bone mineral density with or without bisphosphonate pre-treatment in osteoporosis with rheumatoid arthritis. Arch Osteoporos 12(1):80

  28. Poole KE, Treece GM, Gee AH, Brown JP, McClung MR, Wang A et al (2015) Denosumab rapidly increases cortical bone in key locations of the femur: a 3D bone mapping study in women with osteoporosis. J Bone Miner Res 30(1):46–54

  29. Recknor C, Czerwinski E, Bone HG, Bonnick SL, Binkley N, Palacios S et al (2013) Denosumab compared with ibandronate in postmenopausal women previously treated with bisphosphonate therapy: a randomized open-label trial. Obstet Gynecol 121(6):1291–1299

  30. Reszka AA, Rodan GA (2003) Bisphosphonate mechanism of action. Curr Reumatol Rep 5:65

  31. Rossini M, Adami G, Viapiana O, Ldolazzi L, Gatti D (2016) Denosumab, cortical bone and bone erosions in rheumatoid arthritis. Ann Rheum Dis

  32. Russell RG, Watts NB, Ebetino FH, Rogers MJ (2008) Mechanisms of action of bisphosphonates: similarities and differences and their potential influence on clinical efficacy. Osteoporos Int 19(6):733–759

  33. Schett G, Hayer S, Zwerina J, Redlich K, Smolen JS (2005) Mechanisms of disease: the link between RANKL and arthritic bone disease. Nat Clin Invest 1:47–54

  34. Sinigaglia L, Nervetti A, Mela Q, Bianchi G, Del Puente A, Di Munno O, La Montagna G, Adami S (2000) A multicenter cross sectional study on bone mineral density in rheumatoid arthritis. Italian study group on bone mass in theumatoid arthritis. J Rheumatol 27(11):2582–2589

  35. Sivagurunathan S, Muir MM, Brennan TC, Seale JP, Mason RS (2005) Influence of glucocorticoids on human osteoclast generation and activity. J Bone Miner Res 20(3):390–398

  36. Steinbrocker O, Traeger CH, Batterman RC (1949) Therapeutic criteria in rheumatoid arthritis. J Am Med Assoc 140(8):659–662

  37. Takeuchi T, Tanaka Y, Ishigro N, Yamanaka H, Yoneda T, Ohira T et al (2016) Effect of denosumab on japanese patients with rheumatoid arthritis: a dose-response study of AMG 162 (Denosumab) in patients with rheumatoid arthritis on methotrexate to validate inhibitory effect on bone erosion (DRIVE)-a 12-month, multicenter, randomized, double-blind, placebo-controlled, phase II clinical trial. Ann Rheum Dis 75(6):983–990

  38. Van Staa TP, Leufkens HGM, Cooper C (2002) The epidemiology of corticosteroid-induced osteoporosis: a meta-analysis. Osteoporosis Int 13(10):777–787

  39. Vis M, Havaardsholm EA, Haugeberg G, Uhlig T, Voskuy AE, van de Stadt RJ, Dijkmans BAC et al (2006) Evaluation of bone mineral density, bone metabolism, osteoprotegrin and receptor activator of the NFKB ligand serum levels during treatment with infliximab in patients with rheumatoid arthritis. Ann Rheum Dis 65(11):1495–1499

  40. Wells GA, Cranney A, Peterson J, Boucher M, Shea B, Welch V, Coyle D, Tugwell P (2008a) Alendronate for the primary and secondary prevention of osteoporotic fractures in postmenopausal women. Cochrane Database Syst Rev 1:CD001155

  41. Wells GA, Cranney A, Peterson J, Boucher M, Shea B, Welch V et al (2008b) Risedronate for the primary and secondary prevention of osteoporotic fractures in postmenopausal women. Cochrane Database Syst Rev

  42. Wijbrandts CA, Klaasen R, Dijkgraaf MG, Gerlag DM, van Eck-Smit BL, Tak PP (2009) Bone mineral density in rheumatoid arthritis patients 1 year after adalimumab therapy: arrest of bone loss. Ann Rheum Dis 68(3):373–376

  43. Yasuda H, Shima N, Nakagawa N, Yamaguchi K, Kinosaki M, Mochizuki SI et al (1998) Osteoclast differentiation factor is a ligand for osteoprotegerin/osteoclastogenesis-inhibitory factor and is identical to TRANCE/RANKL. Proc Natl Acad Sci U S A 95(7):3597–3602

Download references

Acknowledgements

The authors would like to thank Hiroshi Inoue, MD for his contribution to acquiring data in this study.

Author information

Conception and design: Drs. TK, KO, and YY. Collection of data and analysis and interpretation of the data: Drs. TK, KO, YY, CO, TS, and MI. Critically revised manuscript: Drs. MT, HS, and HC. Final approval of the article: Drs. TK, KO, YY, CO, TS, MT, HS, MI and HC. All authors read and approved the final manuscript.

Correspondence to Koichi Okamura.

Ethics declarations

Ethics approval and consent to participate

The study was approved by the Institutional Review Board in Gunma University. Appropriate consents, permissions and releases were obtained.

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Kaneko, T., Okamura, K., Yonemoto, Y. et al. Effects of denosumab on bone mineral density and bone turnover markers in rheumatoid arthritis patients switching from bisphosphonates. J EXP ORTOP 6, 41 (2019) doi:10.1186/s40634-019-0211-7

Download citation

Keywords

  • Osteoporosis
  • Denosumab
  • Bisphosphonate
  • Rheumatoid arthritis
  • Fragility fractures