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PET/MRI reveals ongoing metabolic activity in ACL grafts one year post-ACL reconstruction



To use serial PET/MRI imaging to radiographically evaluate the metabolic activity of the ACL graft over the first post-operative year.


Six patients undergoing primary ACL reconstruction were recruited in this prospective study in an inpatient university hospital. All patients underwent femoral and tibial suspensory cortical fixation with quadrupled semitendinosus autograft hamstring ACL reconstruction by an orthopaedic surgeon. Simultaneous 18F-FDG PET and MRI of both the operative and non-operative knee was performed at three, six, and 12 months post-operatively. Quantification of the mean standardized uptake value (SUV) within the whole-knee, as well as tibial tunnel, femoral tunnel, and intra-articular graft regions of interest (ROIs).


PET whole-knee activity was increased at all time-points post-operatively compared to the control, non-operative knee. Activity decreased over time, yet considerable generalized activity remained 1 year post-operatively, with relative intensity 34% percent higher than control. When the operative knee was divided into three whole-regions, there was greater activity in the tibia at three than 12 months, the femur at six than 12 months, and in the tibia compared to the intra-articular region at 3 months. When they were separated into sub-regions, results demonstrated greater activity closer to the joint surface.


PET/MRI evaluation of ACL graft reconstructions demonstrates evolving biologic activity within the graft and both tunnels. Focal areas of increased activity within the tunnels may indicate of ligamento-osseous morphologic changes. These data suggest that graft incorporation continues well beyond 1 year post-operatively.

Level of evidence

Level IV.


The current standard of care for anterior cruciate ligament (ACL) injuries involves the use of quadriceps, bone-patellar tendon-bone, hamstring autograft, or allograft tissues. The biologic incorporation of these grafts has been well-studied using histologic evaluation, animal models, and radiographic assessment. Studies suggest that the tendon graft initially undergoes a process of peripheral synovial vascularization and ultimately achieves ultrastructural incorporation [21, 24]. Post-operative magnetic resonance imaging (MRI) scans of ACL grafts support this notion [16]. However, information is lacking regarding the true biologic and metabolic activity of ACL tendon grafts during the post-operative period.

Another imaging modality, SPECT/CT (Single-photon emission computed tomography/computed tomography), has previously been used with diphosphonate bone tracers to evaluate changes in biomechanical loading and the subchondral bone plate [12]. Higher amounts of this bone tracer uptake has been shown to be significantly related to a higher degree of osteoarthritis in knees post- ACL reconstruction [14]. While this imaging modality serves to measure additional parameters (in vivo joint loading, bone tunnel remodeling and graft incorporation), interpretation is required to differentiate between an inflammatory response versus altered in vivo loading or both [13]. MRI is the gold standard for assessing morphological and structural changes in patients post-ACL reconstruction [17]. Therefore, we chose to combine positron emission tomography (PET), an effective tool for evaluating metabolic activity, with MRI, to demonstrate a more accurate picture of the biological activity within the knee.

18F-FDG PET is a functional imaging technique that uses a radioactive analog of glucose to assess glucose metabolism in body tissues. In addition to its well-established role in oncologic and brain imaging, 18F-FDG PET is also used in the assessment of infection, inflammation, and more recently, tissue repair [7, 10, 19, 23, 25]. 18F-FDG accumulates in regions proportionally to their glycolytic rate, therefore greater uptake is seen in regions of increased metabolic activity. In orthopaedic applications, 18F-FDG-PET has been utilized in the management of patients after arthroplasty and has been effective in evaluating a variety of infectious and inflammatory processes [25, 26].

The feasibility of combined PET/MRI imaging to assess ACL graft healing following surgery has also been investigated, although current research is exploratory. PET has been utilized in combination with MRI to assess FDG uptake of the knee in a canine model of anterior cruciate ligament transection (ACLT) [15]. In this study, five skeletally mature beagles were imaged with PET/CT and MRI pre-operatively and 3, 6, and 12 weeks after ACLT, with the contralateral knee serving as the control. The technique was sensitive to metabolic changes in different structures of the knee joint, suggesting the potential use of FDG as a biomarker for graft viability. In a recent prospective clinical study involving eight patients who underwent ACL graft reconstruction, it was found that 18F-FDG PET could be performed at low radiation burden levels with sufficient image quality to evaluate graft healing [2].

Studies using 18F-FDG PET imaging after ACL reconstruction have demonstrated a significant increase in tendon graft metabolism during the immediate post-operative period, as well as during the first 2 years of post-operative healing [9, 20]. Recently, a study evaluated the utility of 18F-FDG PET /MRI in ligament imaging. This study followed 19 patients post-ACL reconstruction with autografts and allografts at four different time-points from 1.6 months up to 125.9 months [9]. The authors found that 18F-FDG PET/MRI could be used to evaluate metabolic activity of both types of ACL grafts. They also demonstrated that there was a statistically significant decrease in metabolic activity at 2 years post-operatively when compared to earlier time-points.

The purpose of this study was to utilize serial PET/MRI scans to evaluate graft metabolic activity and better understand the process of bio-integration of the ACL graft at precise time-points during the first post-operative year. We hypothesized that early on at the 3-month and 6-month scans there would be increasing biological activity as neovascularization occurs, which would downtrend at the 12-month scan during incorporation of the graft. The timeline of ligamento-osseus changes in the knee post-ACL reconstruction would be helpful in shaping safe return to play parameters in recreational/professional athletes.



Six patients (three males and females) were recruited into this institutional review board (IRB) approved prospective study from 2013 to 2016 (Table 1). This was designed as a pilot study to gain a better understanding of the time-points associated with graft healing and be balanced for sex. All patients underwent all-inside, dual suspensory fixation, quadrupled semitendinosus autograft hamstring ACL reconstruction by one sports fellowship trained orthopedic surgeon. A suspensory type cortical fixation device (ACL Tightrope® RT Arthrex, Naples, FL) was chosen both for its biomechanical characteristics and to allow for improved radiologic evaluation of graft integration within the tunnels without the presence of foreign body fixation. Tunnel widths ranged from 8 mm to 10 mm. Similar amounts of ACL stump were removed with no preservation attempts for all patients at both the femoral and tibial tunnel locations. All patients followed standardized post-operative rehabilitation protocols. Our protocol involves bracing, crutches, ROM exercises, and ice and elevation in the first post-operative week. In week 2, bracing is maintained, and patients weight bear as tolerated, ROM exercises continue, and strengthening is initiated. In week 3, bracing is discontinued and ROM and strengthening exercises are continued. Weeks 4–10 focus on progressive strengthening and neuromuscular control. Weeks 10–16 are the advanced activity phase and add sport-specific exercises. Finally, at 16–22 months patients are returned to all activities. The patients later underwent simultaneous 18F-FDG PET and MRI (Siemens Biograph mMR) of both the operative and non-operative knee (for control) at 3 months, 6 months, and 12 months post-operatively.

Table 1 Patient Demographics

Inclusion criteria for patients were primary unilateral ACL tear, skeletal maturity (age > 18 years), and patients undergoing all-inside, dual suspensory fixation, quadrupled semitendinosus autograft hamstring ACL reconstruction. Exclusion criteria were lateral collateral ligament (LCL), posterior cruciate ligament (PCL), or posterior lateral corner injury, greater than grade two medial collateral ligament (MCL) sprain, multitrauma, skeletal immaturity (age < 18 years), grade three Outerbridge classification changes in one or more compartments, revision case, prior surgery to either knee, ACL insufficient contralateral knee, prisoners, cognitively impaired adults, hospital employees, inability to undergo MRI secondary to non-compatible implantable devices, and females of child-bearing age with positive urine human chorionic gonadotropin (hCG). All patients meeting the criteria were offered inclusion into the study until the male and female groups were filled.

Post-operative imaging

Patient preparation, radiopharmaceutical administration, image acquisition, and analysis followed standard clinical protocols as described below [20]. Patients were instructed to fast four-six hours prior to the intravenous administration of 18F-FDG to decrease physiologic glucose levels and to reduce serum insulin levels to near basal levels. Their blood glucose levels were checked via fingerstick before 18F-FDG administration. Since uptake of 18F-FDG is reduced in hyperglycemic states, the patients were only imaged if their blood glucose levels were less than 150 mg/dL. The patients remained recumbent for 18F-FDG administration and during the subsequent uptake phase to avoid muscular uptake. The recommended dose of 18F-FDG for an adult (70 kg) is 185–370 MBq (5–10 mCi), as an intravenous injection. For this study, we employed 185 MBq. PET scans were started at time of IV injection and were continued for 60 min. Only the last 20 min of the PET uptake data were used, which is equivalent to 40 min of uptake.

After PET acquisition, standard clinical MRI sequences, including sagittal and coronal proton density (PD), sagittal and coronal fat suppressed PD, axial fat suppressed T2 weighted, and additional 3D double echo ultrashort echo time (UTE) were acquired.

Imaging analysis

After image acquisition, whole-knee regions of interest (ROIs) were placed on both knees to characterize changes at the organ scale (Fig. 1). Then, for each tunnel (in both the femur and tibia), MRI images were digitally re-sliced to align the tunnels perpendicular to the image plane and circular ROIs were manually drawn on each plane with diameters equal to the tunnel diameters. The same re-slicing was applied to the corresponding PET images (Fig. 2). The standardized uptake value (SUV), which is a standard PET quantification method, was then calculated for each of these ROIs. The SUV normalized to body weight is given by the following equation: SUV = [ACVOI (kBq/ml)]/[FDG dose (MBq)/BW (kg)], where ACVOI is the attenuation-corrected mean radioactivity concentration value for the volume of interest and Bg is the blood glucose. Although no test-retest data is available specifically for ACL assessment, it’s accuracy is about 20% for tumor imaging when using the mean value over the region of interest, which is more accurate than using the maximum value [5].

Fig. 1
figure 1

Time course of PET uptake following ACL reconstruction. Whole knee PET uptake regions of interest in non-operative (left) and operative (right) knees 3 months, 6 months, and 12 months post-operatively from a single representative patient. Images are taken from axial (top), sagittal (middle), and coronal (bottom) planes centered on the ACL. Increased 18F-FDG uptake is indicated by a shift from blue to red

Fig. 2
figure 2

Realignment of PET/MRI images for quantitative analysis. Images showing the re-sliced PET/MRI images for the tibial tunnel, intra-articular graft, and femoral tunnel. The images were aligned so that the tunnels and graft were perpendicular to the imaging plane on axial (top), sagittal (middle), and coronal (bottom) views. Multiple circular regions of interest matching the tunnel diameters were then positioned on the images (colored overlay) to quantify regional 18F-FDG uptake

In addition, sequential knee MRI images were qualitatively reviewed by a musculoskeletal fellowship trained radiologist for morphological changes or pathology.

IRB approval

This study was approved by Stony Brook University’s IRB (CORIHS B) (FWA #00000125) as CORIHS#2014–2662-F.

Statistical analysis

For statistical analysis, all of the patients were grouped together at each time-point and the data are reported as a group mean ± standard deviation. Total knee SUVs were compared between each patient’s operative and non-operative knee at each time-point using paired t-tests and differences were considered significant for values of p < 0.050. Total knee SUVs were averaged from all patients at each time-point and compared across time using Friedman’s Two-Way Analysis of Variance by ranks, with differences considered significant for values of p < 0.050. For the whole region and subregion SUV, the data were averaged from all patients at each time-point and compared across both region and time using Friedman’s Two-Way Analysis of Variance by ranks, with differences considered significant for values of p < 0.050. All tests were performed using SPSS Ver. 25 (IBM).


Total knee PET activity based on averaged SUV (Fig. 1, Table 2) was increased at all time points in the operative knee when compared to the non-operative knee (3-month, p = 0.000; 6-month, p = 0.001; 12-month, p = 0.006). The activity decreased significantly between the three-month and 12-month time-points in the operative knee (p = 0.022) with the activity at 1 year remaining 34% above that of the contralateral knee.

Table 2 Total Knee Standardized Uptake Value (SUV)

To evaluate the knee at organ-scale, localization was initialized broadly, then advanced to be increasingly specific. When the knees were separated into three distinct whole regions (tibial tunnel, intra-articular graft, femoral tunnel) [Fig. 3], there was a statistically significant difference at the three-month time-point, with increased PET activity in the tibia when compared with the activity within the tibial and intra-articular regions (p = 0.006). When comparing the regions as a function of time, the tibial region had more activity at the six-month time-point than at the 12-month (p = 0.017), while the femoral region had more activity at three- and six-month time-points than at the 12-month (p = 0.000). No differences were seen when comparing the femoral regions as a function of time.

Fig. 3
figure 3

Whole Region SUV. Graph displaying the metabolic activity (y-axis) of the femoral tunnel, intra-articular graft, and tibial tunnel (x-axis). The metabolic activity of the whole regions is differentiated into the three-month (yellow), six-month (purple), and 12-month (brown) time periods. *, $, and # denote significance (p < 0.05) between the tibial tunnel and intra-articular graft, three-month and 12-month time-periods, and 6-month and 12-month time-periods, respectively

When the tunnels and graft were divided into sub-regions (distal, middle and proximal 1/3 of the tibial tunnel, intra-articular graft, and femoral tunnel) [Fig. 4], the tibial tunnel had more activity distally than proximally (p = 0.015) at the six-month time-point. Within the tibial sub-regions, there was more activity in the distal sub-region at the six-month time-point than at the 12-month (p = 0.015). There were no significant differences within the intra-articular graft sub-regions by location or time. Within the femoral tunnel sub-regions, there was more activity distal than proximal at three- (p = 0.006), six- (p = 0.007), and 12-month (p = 0.006) time-points. Proximally, there was more activity at the three-month than at 12-month time-point (p = 0.022). Overall, when observing the activity of the sub-regions, there appeared to more 18F-FDG activity at the surface of the joint. Focal areas of activity were also noted within certain areas of both tunnels and within the intra-articular graft of all patients (Fig. 5).

Fig. 4
figure 4

Subregion SUV. Graph displaying the metabolic activity (y-axis) of the femoral tunnel, intra-articular graft, and tibial tunnel split into proximal, central, and distal subregions (x-axis). An exaggerated sketch of the subregions is overlaid above to correlate with the subregions on the x-axis. The metabolic activity of the subregions is differentiated into the three-month (yellow), six-month (purple), and 12-month (brown) time periods. ^ and + denote significance (p < 0.05) between the distal and proximal subregions and the six-month and 12-month time-periods, respectively

Fig. 5
figure 5

PET imaging shows focal areas of increased uptake. This figure shows three-month, six-month, and 12-month post-operative PET/MRI scans from the same patient scaled to same standardized uptake value (SUV) in the axial (top), sagittal (middle), and coronal (bottom) planes. Focal areas of high 18F-FDG uptake (red) are apparent, as well as an overall decrease in uptake over time


The most important finding in this study is that even a year post-ACL reconstruction, the process of ligamentization of the tendon graft is ongoing compared to the contralateral non-operative knee. The ligamentization and neovascularization process has been well studied in both human and animal models with histologic and advanced imaging protocols [1, 3, 4, 6, 8, 11, 16, 21, 24]. In addition, the biomechanical properties of healing graft tissue have been studied and demonstrated modes of failure that correlate with specific times and remodeling activities [3, 22]. This study adds to the growing literature supporting 18F-FDG PET/MRI as an imaging modality to assess vascularization and incorporation of the graft in ACL reconstruction. With considerable variation between patients, it was important to normalize values to the patient’s own contralateral knee.

Post-operative 18F-FDG PET/MRI evaluations of ACL hamstring graft reconstructions clearly demonstrate evolving biologic activity within the intra-articular region of the graft, as well as the femoral and tibial tunnel fixation points over time. The quantitative decrease in 18F-FDG uptake over time for both the whole knee, the tunnels, and graft confirmed our hypothesis that as the incorporation process occurs there would be a decrease in uptake within the knee. Although this activity decreases over time, considerable generalized activity remains, even at 1 year. This finding points to an ongoing incorporation process that has not resolved at the 1-year time-point. Prior studies have observed distinct morphologic changes within the graft after 1 year and suggested that changes may continue in the graft up to 2 years post-surgery [1, 16, 18, 21]. Continued elevation in generalized PET activity indicates that there is additional biologic information regarding the post-operative reconstructed knee than traditional studies have shown by either MRI or histology.

Of note, there was considerable variation in whole knee 18F-FDG uptake was detected from patient to patient. Our scanning protocol attempted to control for this, and therefore the variability is attributed to each patients’ individual biologic response to surgery. This observation supports our study design to obtain a scan of the normal contralateral knee at each time-point for comparison purposes.

Focal areas of increased and decreased 18F-FDG PET activity were identified within the tibial and femoral tunnels over time (Fig. 5), however there was not enough consistency between samples to allow for quantitative analysis of this phenomenon. Increased PET activity was encountered in both tunnels, particularly near the joint surfaces, and may be indicative of ligamento-osseous morphologic changes. We initially hypothesized that the graft would become increasingly biologically active over time as neovascularization occurs; however, this does not appear to hold true.


Limitations of the study include the small number of patients enrolled. As well, each of the patients elected to participate in this study, creating a potential selection bias. Also, one patient did not complete the scan at the 6-month time-point. The patients were grouped together for analysis to evaluate the effects of the study in a diverse population. Thus, future studies will be powered to identify differences in outcomes between sexes. Given that there was PET activity remaining on the scans at 1 year, those future studies would also benefit from including scans out to 2 years to evaluate for normalization. Comparison of these PET data to SPECT data would also be of benefit. Perhaps more importantly, future studies to correlate PET scans to biomechanical strength will be vital for evaluating return to play for athletes, as well as return to daily activities. PET scans performed with 18F-FDG are known to detect the metabolic activity of inflammatory cells and because of this, some enhanced activity that is seen may be secondary to inflammation versus actual neovascularization. However, one prior study demonstrated that there are no inflammatory cells 3 and 8 weeks post-operatively on second look arthroscopic biopsies of ACL grafts. Another demonstrated no signs of inflammation 7 weeks post-operatively on histologic evaluation of a whole knee specimen. Inherently, while the effect of inflammation in our study is unknown, it is assumed to be marginal [8, 11]. Specific targeting radiopharmaceuticals could be considered to address this issue. Finally, there also is some inherent lack of spatial resolution associated with the acquisition of the PET scan data. Utilizing a higher resolution detector could aid in improving the spatial resolution although it is not known if this would change the results or have any additional significance.


PET/MRI evaluation of ACL graft reconstructions demonstrate evolving biologic activity within the graft and both tunnels. Focal areas of increased activity within the tunnels may be indicative of ligamento-osseous morphologic changes. The operative knee continues to have considerable activity at 1 year out from surgery especially when compared to the contralateral non-operative knee. This suggests ongoing bio-integration of the graft beyond the first post-operative year. With clinical correlation of patient symptoms, measurement of biologic activity within the graft with 18F-FDG PET/MRI can lead to a more detail return to function/play protocol.

Availability of data and materials

All data generated or analyzed during this study are included in this published article.



Anterior Cruciate Ligament


Magnetic Resonance Imaging


Positron Emission Tomography


Anterior Cruciate Ligament Transection


Institutional Review Board


Lateral Collateral Ligament


Posterior Cruciate Ligament


Medial Collateral Ligament


Human Chorionic Gonadotropin


Standardized Uptake Value


Attenuation-corrected mean radioactivity concentration in a volume-of-interest


Body Weight


Proton Density


Ultrashort Echo Time


  1. A. Magnussen R, Binzel K, Zhang J, Wei W, U. Knopp M, C. Flanigan D, E. Hewett T, C. Kaeding C, V. Knopp M (2017) ACL graft metabolic activity assessed by 18FDG PET–MRI. Knee 24(4):792–797.

    Article  PubMed  Google Scholar 

  2. Binzel K, Kaeding C, Flanigan D, Magnussen R, Wei W, Knopp M, Zhang J, Knopp M (2015) Feasibility demonstration of dynamic FDG PET to assess ACL graft viability after reconstructive surgery. J Nucl Med 56(supplement 3):547

    Google Scholar 

  3. Boellaard R, Delgado-Bolton R, Oyen WJ, Giammarile F, Tatsch K, Eschner W, Verzijlbergen FJ, Barrington SF, Pike LC, Weber WA, Stroobants S, Delbeke D, Donohoe KJ, Holbrook S, Graham MM, Testanera G, Hoekstra OS, Zijlstra J, Visser E, Hoekstra CJ, Pruim J, Willemsen A, Arends B, Kotzerke J, Bockisch A, Beyer T, Chiti A, Krause BJ, European Association of Nuclear M (2015) FDG PET/CT: EANM procedure guidelines for tumour imaging: version 2.0. Eur J Nucl Med Mol Imaging 42(2):328–354.

    Article  CAS  PubMed  Google Scholar 

  4. Claes S, Verdonk P, Forsyth R, Bellemans J (2011) The "ligamentization" process in anterior cruciate ligament reconstruction: what happens to the human graft? A systematic review of the literature. Am J Sports Med 39(11):2476–2483.

    Article  PubMed  Google Scholar 

  5. de Langen AJ, Vincent A, Velasquez LM, van Tinteren H, Boellaard R, Shankar LK, Boers M, Smit EF, Stroobants S, Weber WA, Hoekstra OS (2012) Repeatability of 18F-FDG uptake measurements in tumors: a metaanalysis. J Nucl Med 53(5):701–708.

    Article  CAS  PubMed  Google Scholar 

  6. Falconiero RP, DiStefano VJ, Cook TM (1998) Revascularization and ligamentization of autogenous anterior cruciate ligament grafts in humans. Arthroscopy 14(2):197–205.

    Article  CAS  PubMed  Google Scholar 

  7. Fernandez P, de Clermont-Gallerande H, Dauchy F, Massaloux K, Dupon M (2013) Imagerie scintigraphique de l’infection des prothèses de hanche et de genou. Médecine Nucléaire 37(8):353–361.

    Article  Google Scholar 

  8. Ge Y, Li H, Tao H, Hua Y, Chen J, Chen S (2015) Comparison of tendon-bone healing between autografts and allografts after anterior cruciate ligament reconstruction using magnetic resonance imaging. Knee Surg Sports Traumatol Arthrosc 23(4):954–960.

    Article  PubMed  Google Scholar 

  9. Itala A, Alihanka S, Kosola J, Kemppainen J, Ranne J, Kajander S (2016) Tendon graft healing in multiligament reconstructed knee detected by FDG-PET/CT: a pilot study. Scand J Surg 105(2):133–138.

    Article  CAS  PubMed  Google Scholar 

  10. Keidar Z, Nitecki S (2013) FDG-PET in prosthetic graft infections. Semin Nucl Med 43(5):396–402.

    Article  PubMed  Google Scholar 

  11. Lazarides AL, Eward WC, Green K, Cardona DM, Brigman BE, Taylor DC (2015) Histological evaluation of tendon-bone healing of an anterior cruciate ligament hamstring graft in a 14-year-old boy. Am J Sports Med 43(8):1935–1940.

    Article  PubMed  Google Scholar 

  12. Mathis DT, Buel L, Rasch H, Amsler F, Hirschmann MT, Hugli RW (2019) Distribution of bone tracer uptake in symptomatic knees after ACL reconstruction compared to asymptomatic non-operated knees: a method for better differentiating patient-specific from disease-specific bone tracer uptake in SPECT/CT. Ann Nucl Med 33(3):201–210.

    Article  PubMed  Google Scholar 

  13. Mathis DT, Hirschmann A, Falkowski AL, Kiekara T, Amsler F, Rasch H, Hirschmann MT (2018) Increased bone tracer uptake in symptomatic patients with ACL graft insufficiency: a correlation of MRI and SPECT/CT findings. Knee Surg Sports Traumatol Arthrosc 26(2):563–573.

    Article  PubMed  Google Scholar 

  14. Mathis DT, Rasch H, Hirschmann MT (2015) In vivo bone tunnel remodeling in symptomatic patients after ACL reconstruction: a retrospective comparison of articular and extra-articular fixation. Muscles Ligaments Tendons J 5(4):316–324.

    Article  PubMed  Google Scholar 

  15. Menendez M, Clark D, Binzel K, Hettlich B, Knopp M (2015) F-18-sodium fluoride (NAF) PET-CT and co-registered MRI knee assessment after ACL transection in an in vivo canine model. Osteoarthr Cartil 23:A252–A253.

    Article  Google Scholar 

  16. Ntoulia A, Papadopoulou F, Zampeli F, Ristanis S, Argyropoulou M, Georgoulis A (2013) Evaluation with contrast-enhanced magnetic resonance imaging of the anterior cruciate ligament graft during its healing process: a two-year prospective study. Skelet Radiol 42(4):541–552.

    Article  Google Scholar 

  17. Papakonstantinou O, Chung CB, Chanchairujira K, Resnick DL (2003) Complications of anterior cruciate ligament reconstruction: MR imaging. Eur Radiol 13(5):1106–1117.

    Article  PubMed  Google Scholar 

  18. Rougraff BT, Shelbourne KD (1999) Early histologic appearance of human patellar tendon autografts used for anterior cruciate ligament reconstruction. Knee Surg Sports Traumatol Arthrosc 7(1):9–14.

    Article  CAS  PubMed  Google Scholar 

  19. Saleem BR, Pol RA, Slart RH, Reijnen MM, Zeebregts CJ (2014) 18F-Fluorodeoxyglucose positron emission tomography/CT scanning in diagnosing vascular prosthetic graft infection. Biomed Res Int 2014:471971.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Sorensen J, Michaelsson K, Strand H, Sundelin S, Rahme H (2006) Long-standing increased bone turnover at the fixation points after anterior cruciate ligament reconstruction: a positron emission tomography (PET) study of 8 patients. Acta Orthop 77(6):921–925.

    Article  PubMed  Google Scholar 

  21. Unterhauser FN, Bail HJ, Hoher J, Haas NP, Weiler A (2003) Endoligamentous revascularization of an anterior cruciate ligament graft. Clin Orthop Relat Res 414:276–288.

    Article  Google Scholar 

  22. Weiler A, Peters G, Maurer J, Unterhauser FN, Sudkamp NP (2001) Biomechanical properties and vascularity of an anterior cruciate ligament graft can be predicted by contrast-enhanced magnetic resonance imaging. A two-year study in sheep. Am J Sports Med 29(6):751–761.

    Article  CAS  PubMed  Google Scholar 

  23. Yue B, Tang T (2015) The use of nuclear imaging for the diagnosis of periprosthetic infection after knee and hip arthroplasties. Nucl Med Commun 36(4):305–311.

    Article  PubMed  Google Scholar 

  24. Zaffagnini S, De Pasquale V, Marchesini Reggiani L, Russo A, Agati P, Bacchelli B, Marcacci M (2007) Neoligamentization process of BTPB used for ACL graft: histological evaluation from 6 months to 10 years. Knee 14(2):87–93.

    Article  CAS  PubMed  Google Scholar 

  25. Zhuang H, Codreanu I (2015) Growing applications of FDG PET-CT imaging in non-oncologic conditions. J Biomed Res 29(3):189–202.

    Article  PubMed  PubMed Central  Google Scholar 

  26. Zhuang H, Yang H, Alavi A (2007) Critical role of 18F-labeled Fluorodeoxyglucose PET in the Management of Patients with Arthroplasty. Radiol Clin N Am 45(4):711–718.

    Article  PubMed  Google Scholar 

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Yulia Kulyomina created the artwork in Fig. 2.


This project was supported by a grant from the Stony Brook University Department of Radiology.

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SK substantially contributed in acquiring data, designing the project, and analyzing and interpreting the data. MS substantially contributed in the conception and design of the study. JA substantially contributed in revision of the drafts, and analysis and interpretation of data. MH substantially contributed in the conception and design of the study. PV substantially contributed in the conception and design of the study. AP substantially contributed in the analysis of the study. DK substantially contributed to the conception, design, interpretation of data, and revision of the drafts. JP substantially contributed in the conception and design of the study. All authors read and approved the final manuscript.

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Correspondence to David E. Komatsu.

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The Stony Brook University IRB approved this study, and the patients were consented to participate.

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Competing interests

James M. Paci serves as educational consultant for and has received research support from Arthrex Inc.

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Korbin, S., Salerno, M., Achonu, J.U. et al. PET/MRI reveals ongoing metabolic activity in ACL grafts one year post-ACL reconstruction. J EXP ORTOP 7, 40 (2020).

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