Fig. 2

A Immunocytochemical detection of intracellular COL1 production (green) and SMA-positive stress fibers (red) increase in a dose-dependent manner 48 h after inoculation of unstimulated (US) HFLS with recombinant TGFβ1. Bar = 25 μm (B) Changes in proliferation rate between samples that remained non-treated (NT) and those stimulated with TGFβ1 and treated with MXD individually or in combination was measured by immunocytochemistry of Ki67-positive (red) nuclei over total DAPI (gray)-stained nuclei. Bar = 25 μm (C) Expression of target genes measured by qPCR from RNA extracted from HFLS treated under various conditions for 8-days was analyzed by ΔΔCt method and log scaled following statistics. D, E COL1 and Pyd output were measured by sandwich and competitive ELISA, respectively, from homogenized HFLS in the presence and absence of MXD and/or TGFβ1. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001, no asterisk: p > 0.05