Fig. 2

Minced cartilage + bone chips + fibrin, no digestion with collagenase (Well 4): Positive staining with S-100 for chondrocytes situated in the lacunas, no chondrocytes seen outside the cartilage pieces (marked with black arrow). Cell elements with extracellular matrix beneath the cartilage piece positioned centrally in the figure. These cells weren`t stained with S-100 and supposedly originate from the bone chips, i.e. bone-marrow derived cells (marked with white arrow). In the upper corner porcine epidermis with no cell elements (marked with red arrow) can be seen. No adhesion of the newly formed matrix with the piece of cartilage