Impact of mechanical stimulation on the chondrogenic processes in human bone marrow aspirates modified to overexpress sox9 via rAAV vectors

Journal of Experimental Orthopaedics

Table 4 Histomorphometric analyses in the rAAV-transduced human bone marrow aspirates (day 28)

Assay	Static culture			Dynamic culture
Assay	no vector	luc	sox9	no vector	luc	sox9
Safranin O staining	1.8 (0.2)	1.8 (0.3)	3.3 (0.2)^a,b	1.8 (0.2)	2.0 (0.2)	4.0 (0.1)^a,b,c
Type-II collagen immunostaining	2.2 (0.2)	2.2 (0.1)	3.1 (0.2)^a,b	2.4 (0.2)	2.5 (0.1)	3.6 (0.2)^a,b,c
Alizarin red staining	3.2 (0.2)	3.0 (0.3)	0.9 (0.3)^a,b	3.5 (0.1)	3.4 (0.2)	0.7 (0.1)^a,b
Type-X collagen immunostaining	3.0 (0.2)	3.1 (0.1)	0.8 (0.2)^a,b	3.0 (0.2)	3.1 (0.2)	0.6 (0.1)^a,b

Values are given as mean (SD) with n = 4 independent samples tested in triplicate per vector treatment and culture condition in three independent experiments. Safranin O and alizarin red staining and type-II and -X collagen immunostaining were scored as described in Table 5 (Rey-Rico et al. 2015b). Statistically significant relative to ^ano vector treatment, ^brAAV-luc application, and ^cstatic culture