Assay | Static culture | Dynamic culture | ||||
---|---|---|---|---|---|---|
no vector | luc | sox9 | no vector | luc | sox9 | |
Safranin O staining | 0.3 (0.2) | 0.5 (0.2) | 2.4 (0.3)a,b | 1.8 (0.3)c | 1.9 (0.3)c | 3.1 (0.2)a,b,c |
Type-II collagen immunostaining | 2.1 (0.1) | 2.2 (0.2) | 3.2 (0.1)a,b | 2.7 (0.2)c | 2.6 (0.2)c | 3.8 (0.2)a,b,c |
Alizarin red staining | 3.3 (0.3) | 3.1 (0.4) | 0.8 (0.4)a,b | 3.2 (0.1) | 3.2 (0.3) | 0.7 (0.2)a,b |
Type-X collagen immunostaining | 3.2 (0.2) | 3.0 (0.1) | 0.7 (0.2)a,b | 3.1 (0.4) | 3.0 (0.2) | 0.8 (0.1)a,b |