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Fig. 5 | Journal of Experimental Orthopaedics

Fig. 5

From: Effects of rAAV-mediated FGF-2 gene transfer and overexpression upon the chondrogenic differentiation processes in human bone marrow aspirates

Fig. 5

Gene expression analyses by real-time RT-PCR in rAAV-transduced, chondrogenically-induced human bone marrow aspirates overexpressing FGF-2. The aspirates were transduced with rAAV-lacZ or rAAV-hFGF-2 as described in the Figs. 1, 2, 3, and 4 and kept in chondrogenic medium for 21 days. The samples were processed to monitor the expression profiles of aggrecan (ACAN), type-II collagen (COL2A1), the transcription factor SOX9, type-I collagen (COL1A1), type-X collagen (COL10A1), matrix metalloproteinase 13 (MMP13), alkaline phosphatase (ALP), and the transcription factor RUNX2, with GAPDH serving as a housekeeping gene and internal control for normalization. Ct values were generated for each target gene and fold inductions (relative to rAAV-lacZ-treated aspirates) were measured by using the 2-ΔΔCt method as described in the Methods. *Statistically significant compared with rAAV-lacZ

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