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Fig. 3 | Journal of Experimental Orthopaedics

Fig. 3

From: Effects of rAAV-mediated FGF-2 gene transfer and overexpression upon the chondrogenic differentiation processes in human bone marrow aspirates

Fig. 3

Effects of FGF-2 overexpression upon the production of cartilage-specific components in rAAV-transduced, chondrogenically-induced human bone marrow aspirates. The aspirates were transduced with rAAV-lacZ or rAAV-hFGF-2 as described in the Figs. 1 and 2 and kept in chondrogenic medium for 21 days. The samples were processed to monitor the deposition of proteoglycans and of type-II collagen (toluidine blue staining and immunodetection, respectively) and the expression of SOX9 (immunodetection) (all at magnification ×20; representative data) with corresponding histomorphometric assessments and to estimate the proteoglycan and type-II collagen contents as described in the Methods. *Statistically significant compared with rAAV-lacZ

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