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Figure 2 | Journal of Experimental Orthopaedics

Figure 2

From: Induction of bone formation in abdominal implants constituted by collagen sponges embedded with plant-based human transforming growth factor family proteins in ectopic dog model

Figure 2

Identification by western-blot of recombinant BMP-2, BMP-7 and TGF-β1 from Nicotiana benthamiana leaves. Crude extracts from plants containing each factor and obtained as described in the text were filtered on 0.2 μm membranes and loaded on a HisTrap HP column. Each factor was loaded separately. Fractions were dialyzed against PBS, loaded on a SDS-PAGE gel, transferred to nitrocellulose membranes and incubated with specific rabbit policlonal antibodies to confirm its identity. Molecular weight markers (kDa) are indicated on the left.

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