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Fig. 4 | Journal of Experimental Orthopaedics

Fig. 4

From: Successful isolation of viable stem cells from cryopreserved microfragmented human adipose tissue from patients with knee osteoarthritis – a comparative study of isolation by tissue explant culture and enzymatic digestion

Fig. 4

Alizarin Red S staining of osteogenic induced and non-induced cells. (A) Representative image of monolayer stem cells from cryopreserved microfragmented adipose tissue when isolated by tissue explant culture and cultured in osteogenic induction medium or normal expansion medium (negative control) in 6-wells for 21 days and stained with Alizarin Red S. (B) Box plot of Alizarin Red S concentration [mM] of osteogenic induced and non-induced paired stem cells from cryopreserved microfragmented adipose tissue when isolated with tissue explant culture (TEC) and enzymatic digestion (ED), respectively. ns: non-significant, *: p < 0.05, ***: p < 0.001 when analyzed with paired t-tests. (C) Phase contrast image (20x) of stem cells from cryopreserved microfragmented adipose tissue when isolated with TEC and stained with Alizarin Red S after 21 days of culture in osteogenic induction medium. Red color shows calcium deposits, and (D) after 21 days of culture in normal expansion medium (negative control). Size bars show 1000 µm

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