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Journal of Experimental Orthopaedics
Fig. 3 | Journal of Experimental Orthopaedics

Fig. 3

From: Successful isolation of viable stem cells from cryopreserved microfragmented human adipose tissue from patients with knee osteoarthritis – a comparative study of isolation by tissue explant culture and enzymatic digestion

Fig. 3

Oil-Red-O staining of adipogenic induced and non-induced cells. (A) Box plot of Oil-Red-O concentration (%) of adipogenic induced and non-induced paired stem cells from cryopreserved microfragmented adipose tissue when isolated with tissue explant culture (TEC) and enzymatic digestion (ED), respectively. ns: non-significant, **: p < 0.01 when analyzed with paired t-tests. (B) Phase contrast image (20x) of stem cells from cryopreserved microfragmented adipose tissue when isolated with TEC and stained with Oil-Red-O after 14 days of culture in adipogenic induction medium. Red color shows lipid vacuoles (arrows). (C) Phase contrast image (20x) of stem cells from cryopreserved microfragmented adipose tissue when isolated with TEC and stained with Oil-Red-O after 14 days of culture in normal expansion medium (negative control). Size bars show 500 µm

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