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Fig. 1 | Journal of Experimental Orthopaedics

Fig. 1

From: Successful isolation of viable stem cells from cryopreserved microfragmented human adipose tissue from patients with knee osteoarthritis – a comparative study of isolation by tissue explant culture and enzymatic digestion

Fig. 1

Viability and cell culture. Flow cytometry data of a LIVE/DEAD® Viability/Cytotoxicity Kit for mammalian cells. (A) Gating of stem cells based on side scatter (SSC-A) and forward scatter (FSC-A) to exclude debris and leucocytes, and (B) Sample staining showing gating on live and dead cells based on green and red fluorescence following isolation with enzymatic digestion (ED) immediately after thawing. (C) Unstained control showing no false positive on the flow cytometry viability assay. Box plot showing (D) viable cumulative population doublings (cPDs) and (E) doubling time (DT) (in days) of stem cells at passage (P) 1 to 3 from cryopreserved microfragmented adipose tissue (AT) (n = 7) when isolated with tissue explant culture (TEC) and ED, respectively. Error bars indicate standard deviation. (F) Representative brightfield images at 20 × magnification of paired monolayer cells at passage 4 when isolated with TEC and ED, respectively, and cultured in normal expansion medium

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