Systematic review on the application of 3D-bioprinting technology in orthoregeneration: current achievements and open challenges

Pan, Rachel L.; Martyniak, Kari; Karimzadeh, Makan; Gelikman, David G.; DeVries, Jonathan; Sutter, Kelly; Coathup, Melanie; Razavi, Mehdi; Sawh-Martinez, Rajendra; Kean, Thomas J.

doi:10.1186/s40634-022-00518-3

Journal of Experimental Orthopaedics

Table 5 Vascularized bone 3D bioprinting: materials, cells and characterizations

From: Systematic review on the application of 3D-bioprinting technology in orthoregeneration: current achievements and open challenges

First author	Materials	Cells	Material Characterization	In vitro tests	In vivo assay	Ref #
Byambaa	GelMA, silicate nanoplatelets	Human MSCs, HUVECs	Mechanical, degradation	Viability, proliferation, vasculogenic potential, qPCR, Alizarin red, immunohistochemistry		[27]
Chen	PDACS polydopamine modified calcium silicate, PCL, alginate, gelatin	Wharton's Jelly-derived MSCs, HUVECs	Swelling	Mitochondrial activity assay, viability, microscopy, biochemistry		[33]
Cidonio	Laponite, GelMA	Human BMSCs	Rheology, degradation, swelling, mechanical, lysozyme resorption + release	Viability, proliferation, histology	Chick chorioallantoic membrane model	[35]
Daly	GelMA, pluronic F127	Rat MSCs	Mechanical	Histology, immunohistochemistry, viability, biochemistry	Femoral defects rats	[46]
Freeman	RGD alginate, methylcellulose, PCL	Pig BMSCs	Growth factor release, rheology	Biochemistry	Femoral defects rats	[64]
Kuss	PCL, hydroxyapatite, HAMA, GelMA	pig stromal vascular fraction cells		Viability, mineralization, qPCR	Subcutaneous in nude mice	[102]
Lee	Hyaluronic acid derivatives (acrylated HA and tyramine-conjugated HA)	Mouse fibroblast cells (L929) and Human MSCs	Rheology	Viability, qPCR		[106]
Leucht	GelMA, hydroxyapatite, acetylated gel, gelatin	Human adipose derived stromal cells, human dermal microvascular endothelial cells	Mechanical, swelling, sol–gel transition, gelation points	Immunofluorescence		[108]
Nulty	Fibrinogen, hyaluronic acid, hydroxyapatite, type A gelatin, RGD alginate, PCL	HUVECs, Human BMSCs	SEM, degradation	Viability	Femoral defects rats	[141]
Park	Type I collagen, PCL, alginate, gelatin	Human dental pulp stem cells (hDPSCs), Human MSCs		Viability, qPCR	Subdermal implants into balb/c mice	[148]
Piard	Fibrinogen, gelatin	HUVECs, Human MSCs	Swelling, rheology, SEM, mechanical	Viability, qPCR, DNA quantification	Subdermal in rats	[151]
Yang	Type I collagen, GelMA, fibrin	Human BMSCs, iPSCs, bone marrow MSCs		F-actin staining, microscopy, bead aggregation assay, qPCR, histology		[196]

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