Systematic review on the application of 3D-bioprinting technology in orthoregeneration: current achievements and open challenges

Pan, Rachel L.; Martyniak, Kari; Karimzadeh, Makan; Gelikman, David G.; DeVries, Jonathan; Sutter, Kelly; Coathup, Melanie; Razavi, Mehdi; Sawh-Martinez, Rajendra; Kean, Thomas J.

doi:10.1186/s40634-022-00518-3

Journal of Experimental Orthopaedics

Table 4 Osteochondral 3D bioprinting: materials, cells and characterizations

From: Systematic review on the application of 3D-bioprinting technology in orthoregeneration: current achievements and open challenges

First author	Materials	Cells	Material Characterization	In vitro tests	In vivo assay	Ref #
Ahlfeld	Calcium phosphate cement, alginate-methylcellulose	Human MSCs	SEM, mechanical	Viability, microscopy		[4]
Critchley	PCL, PLA, PLGA, alginate, agarose	Porcine or goat BMSCs, chondrocytes, adipose derived stromal cells	Mechanical	Histology, immunohistochemistry	Subcutaneous in nude mice	[39]
Deng	Silk fibroin-methacrylic anhydride, GelMA, LAP	Rabbit articular chondrocytes, rabbit BMSCs	Rheology, mechanical, degradation, microscopy	Immunofluorescence, viability, qPCR, biochemistry	Osteochondral defect in rabbit	[51]
Fedorovich	Alginate, calcium phosphate particles	Human MSCs, human articular chondrocytes	Rheology, mechanical	Viability, fluorescence, immunohistochemistry	Subcutaneous in nude mice	[60]
Kang	PCL, pluronic f127, tricalcium phosphate	Human adipose derived stromal cells		Viability, histology		[89]
Kilian	Calcium phosphate cement (CPC), alginate-methylcellulose,	Human articular chondrocytes		Viability, immunofluorescence, qPCR, medium composition analysis		[95]
Levato	PLA, GelMA, type I collagen	Rat MSCs	Mechanical	Biochemistry, viability, histology, immunofluorescence		[109]
Moses	Silk fibroin, hydroxyapatite, polyvinylpyrrolidone	Pig adipose derived stromal cells	Rheology, light scattering, XRD	Viability, biochemistry, immunohistochemistry, tube formation assay	Implanted using insulin syringe, not bioprinted sub-cutaneous implant	[131]
Schuurman	PCL, alginate	Human articular chondrocytes	Mechanical	Viability		[165]
Shim	Hyaluronic acid, atelocollagen, PCL	Human turbinate-derived MSCs	SEM	Viability, immunofluorescence, qPCR, histology	Patellar defect in rabbits	[169]

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