Systematic review on the application of 3D-bioprinting technology in orthoregeneration: current achievements and open challenges

Pan, Rachel L.; Martyniak, Kari; Karimzadeh, Makan; Gelikman, David G.; DeVries, Jonathan; Sutter, Kelly; Coathup, Melanie; Razavi, Mehdi; Sawh-Martinez, Rajendra; Kean, Thomas J.

doi:10.1186/s40634-022-00518-3

Journal of Experimental Orthopaedics

Table 2 Bone 3D bioprinting: materials, cells and characterizations

From: Systematic review on the application of 3D-bioprinting technology in orthoregeneration: current achievements and open challenges

First author	Materials	Cells	Material Characterization	In vitro tests	In vivo assay	Ref #
Ahlfeld	Laponite-alginate-methylcellulose	Human hTERT-MSCs	Rheology, swelling, SEM, mechanical	Viability, biochemistry		[3]
Amler	GelMA	Human myogenic precursor cells		Viability, metabolic activity, mineralization, qPCR		[7]
Antich	Hyaluronic acid-alginate, PLA	Human articular Chondrocytes	Rheology, degradation, mechanical	Mechanical, viability, proliferation, karyotyping, biochemical, qPCR		[11]
Armstrong	Alginate pluronic hybrid	Human BMSCs	Rheology, spectroscopy, mechanical, SEM, calcium depletion test	Viability, histology		[12]
Bendtsen	Alginate-phosphate, hydroxyapatite-PVA/Ca sulphate	Mouse calvaria 3T3-E1 (MC3T3)	Rheology, degradation, mechanical	Viability		[19]
Bertassoni	GelMA, SPELA PEGDMA and PEGDA and agarose template	Mouse calvarial pre-osteoblasts cells (MC3T3)	Mechanical, swelling	Viability, mineralization, immunohistochemistry, microscopy		[20]
Breathwaite	Scaffold free	Human BM-MSC		Mineralization, qPCR, histology		[24]
Byambaa	GelMA, silicate nanoplatelets	Human MSCs, HUVECs	Mechanical, degradation	Viability, proliferation, vasculogenic potential, qPCR, Alizarin red, immunohistochemistry		[27]
Carlier	Agarose	Mouse fibroblasts		Viability, microscopy		[29]
Cunniffe	RGD-γ-irradiated alginate and nano-hydroxyapatite (nHA) complexed to plasmid DNA (pDNA)	Human MSCs		Viability, biochemistry, mineralization	Subcutaneous in nude mice	[43]
Dubey	Extracellular matrix/amorphous magnesium phosphates hydrogel	Human dental pulp stem cells (hDPSCs)	SEM, rheology, spectroscopy (FTIR)	Viability, microscopy, mineralization, qPCR	Cranial defects in rats	[55]
Emmermacher	Alginate, methylcellulose and agarose (AMA)	Human hTERT-MSCs and plant cell culture of basil	Rheology	Viability		[57]
Filardo	Collagen-based hydrogel	Human MSCs		Viability		[61]
Guduric	Alginate-methylcellulose blend with mesoporous bioactive glasses	Human hTERT-MSCs	TEM, rheology, mass flow	DNA Quantification, ion release		[73]
Gurkan	GelMA	Human MSCs		qPCR		[74]
Hernández-Tapia	Alginate-gelatin	Human osteoblastic cell lines	SEM	Viability, metabolic activity, mineralization, microscopy		[79]
Kim	GelMA	MSCs		Viability, microscopy	Calvarial defects in rats	[96]
Lee	Hyaluronic acid derivatives (acrylated HA and tyramine-conjugated HA)	Mouse fibroblast cells (L929) and Human MSCs	Rheology	Viability, qPCR		[106]
Levato	GelMA	Equine ACPCs, articular chondrocytes, MSCs		Viability, morphology, biochemistry, qPCR, mechanical		[110]
Liu	Alginate-gelatin composite hydrogels	Mouse MSCs	Rheology, SEM, porosity	Viability, adhesion, qPCR, histology		[114]
Maturavongsadit	Nanocellulose/ chitosan	Mouse pre-osteoblast cell line (MC3T3-E1 cells)	Rheology, SEM, swelling, mechanical	Viability, qPCR, biochemistry, mineralization, histology		[123]
Montheil	Silylated hybrid hydroxypropyl methyl cellulose hydrogel	Human MSCs	Thermogravimetric analysis, rheology, swelling, SEM, NMR	Viability		[129]
Moore	Methylcellulose and alginate	Human MSCs & endothelial cells	Rheology, SEM	Viability, flow cytometry, biochemistry, histology, hypoxia assessment		[130]
Murphy	PCL/borate glass composite	Human adipose derived stromal cells	SEM, degradation, spectroscopy (EDX)	Viability		[134]
Park	GelMA	Human dental pulp stem cells (hDPSCs)	Rheology, mechanical	Viability, proliferation, histology, qPCR		[147]
Rukavina	Fibrinogen and Osteo-hydrogel (Fibrin/Gelatin/ Hyaluronic/ Glycerol)	Human adipose derived stromal cells and HUVECs		Immunohistochemistry, Histology	Subcutaneous in nude mice	[163]
Schwab	Tyramine derivative of hyaluronan (THA) and Col 1	Human MSCs	Rheology, spectrometry	Viability, cell migration, histology, qPCR, immunohistochemistry, biochemistry		[167]
Sun	GelMA/gelatin/PEG/ mesoporous silica nanoparticles composite	Rat BMSCs	Rheology, mechanical, TEM/SEM	Viability, collagenase test, qPCR, immunohistochemistry, histology	Calvarial defects of DM rats	[178]
Wang	GelMA/HAMA	Mouse MSCs	SEM/TEM, doxycycline release assay	Viability, proliferation, antibacterial test, histology, qPCR	Subcutaneous in nude mice	[191]
Wang	Alginate-gelatin hydrogel	Human osteogenic sarcoma cells	SEM	Viability, mineralization, biochemistry		[192]
Yang	GelMA and HAMA, type I collagen	Mouse osteocyte cell line (IDG-SW3)	Rheology, SEM, mechanical	Viability, mineralization, immunocytochemistry, qPCR, histology		[199]
Zhai	Polyethylene glycol diacrylate/hyaluronic acid	Rat osteoblasts	Spectroscopy, mechanical, ion leaching, SEM	Viability, proliferation, morphology, histology	Tibia bone defects in rats	[204]
Zhang	Alginate/gelatin with graphene oxide	Human MSCs	TEM, rheology, mechanical	Viability, histology, uCT		[205]
Zhang	Alginate/gelatin	Human MSCs	Rheology, mechanical	Viability, qPCR, histology, uCT		[206]
Zheng	Silk/PEG Bioink	Human MSCs	Rheology, spectroscopy (FTIR)	Viability, DNA quantification	Subcutaneous in mice	[211]

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