Systematic review on the application of 3D-bioprinting technology in orthoregeneration: current achievements and open challenges

Pan, Rachel L.; Martyniak, Kari; Karimzadeh, Makan; Gelikman, David G.; DeVries, Jonathan; Sutter, Kelly; Coathup, Melanie; Razavi, Mehdi; Sawh-Martinez, Rajendra; Kean, Thomas J.

doi:10.1186/s40634-022-00518-3

Journal of Experimental Orthopaedics

Table 1 Cartilage 3D bioprinting: materials, cells and characterizations

From: Systematic review on the application of 3D-bioprinting technology in orthoregeneration: current achievements and open challenges

First author	Materials	Cells	Material Characterization	In vitro tests	In vivo assay	Ref #
Antich	Hyaluronic acid-alginate, PLA	Human articular chondrocytes	Rheology, degradation, mechanical	Mechanical, viability, proliferation, karyotyping, biochemical, qPCR		[11]
Armstrong	Alginate pluronic hybrid	Human BMSCs	Rheology, spectroscopy, mechanical, SEM, calcium depletion test	Viability, histology		[12]
Costantini	GelMA, HAMA, chondroitin sulfate amino ethyl methacrylate, alginate, I2959	Human BMSCs	Rheology, micro-computed tomography, mechanical	Viability, immunocytochemistry, qPCR,		[38]
Cui	PEGDMA, I2959	Human articular chondrocytes	Mechanical, swelling	Viability, qPCR, biochemistry, histology		[42]
Cui	PEGDMA, I2959	Human Articular Chondrocytes	Mechanical, swelling	Viability, qPCR, biochemistry, histology		[41]
Daly	Agarose, alginate, GelMA, PEGMA, PCL	Pig BMSCs		Viability, mechanical		[44]
Daly	RDG-y Alginate, GelMA, PEGMA, PCL	Pig BMSCs		Biochemistry, histology, viability, uCT, mechanical	Subcutaneous in nude mice	[45]
Fan	GelMA, HAMA, cellulose nanocrystals, LAP	ATDC5s	Rheology, mechanical, swelling, printability	Viability		[59]
Galarraga	norbornene-modified hyaluronic acid, LAP	Bovine BMSC	Rheology	Viability, qPCR, mechanical, biochemical, histology		[66]
Gao	PEGDA, acrylated peptides, I2959	Human BMSCs	Mechanical, swelling	qPCR, biochemistry, histology	Subcutaneous in nude mice	[67]
Gatenholm	Nanocellulose, alginate, calcium chloride crosslinking	Human articular chondrocytes		Histology, microscopy, qPCR		[69]
Goldstein	Type I collagen, alginate	Rat articular chondrocytes		Biochemistry, qPCR		[70]
Graham	Agarose, collagen	HEK, ovine MSCs	Gelation, phase transfer	Viability, immunocytochemistry, histochemistry, qPCR		[71]
Gu	GelMA, I2959	Human articular chondrocytes	Rheology, mechanical	Proliferation, viability		[72]
Hauptstein	Thiol-modified hyaluronic acid, P(AGE-co-G), I2959, PCL	Human BMSC		Viability, histology, immunohistochemistry, biochemical, qPCR, mechanical, swelling, SEM		[76]
Henrionnet	Gelatin, alginate, fibrinogen	Human BMSC		Mitochondrial activity, qPCR, histology, biochemistry		[78]
Huang	Yeast Mannan, methacrylate anhydride, I2959, LAP	Rabbit articular chondrocytes	Rheology, SEM, swelling, degradation, mechanical	Viability	Subcutaneous in nude mice	[80]
Ilhan	Kappa carrageenan-methacrylate, I2959	ATDC5s	Rheology, biodegradation, swelling	Viability, proliferation, morphology, immunohistochemistry, qPCR, biochemistry		[82]
Irmark	GelMA, I2959, platelet rich plasma	ATDC5s	Rheology, biodegradation, platelet activation, growth factor release	Viability, proliferation, morphology, immunohistochemistry, qPCR, biochemistry		[84]
Izadifar	PCL, alginate,	Chicken articular chondrocytes, ATDC5	Viscosity, biocompatibility	Viability, proliferation, differentiation		[85]
Kessel	HAMA, GelMA, hyaluronic acid transglutaminase, LAP	C2C12 Myocytes, Bovine articular chondrocytes	Rheology, mechanical, swelling	Viability, histology, immunohistochemistry,		[93]
Kim	Oxidized hyaluronate, glycol chitosan, adipic acid dihydrazide	ATDC5s	Assessment of self-healing	Viability, qPCR		[97]
Kosik-Koziol	PLA, alginate	Human articular chondrocytes	Rheology, SEM, mechanical, swelling	Viability		[100]
Kosik-Koziol	GelMA, alginate, β-tricalcium phosphate particles	Human BMSC	Rheology, SEM, swelling, mechanical	Immunocytochemistry, viability, qPCR		[101]
Lam	GelMA, HAMA, LAP	Pig articular chondrocytes		Histology, viability, qPCR		[104]
Levato	GelMA, I2959	Equine ACPCs, articular chondrocytes, MSCs		Viability, morphology, biochemistry, qPCR, mechanical		[110]
Lim	Methacrylated poly(vinyl alcohol) (PVA-MA), GelMA, RU/SPS photoinitiator	Human MSCs	Viscosity, swelling, mechanical	Viability		[112]
López-Marcial	Agarose, alginate	Bovine articular chondrocytes	Rheology, mechanical	Viability, biochemistry		[117]
Luo	GelMA, LAP	Rat BMSC	Rheology, mechanical, SEM	Viability, morphology, proliferation, biochemistry, qPCR	SCID mice, intramuscular	[118]
De Moor	GelMA, I2959 or LAP	Human BMSC	Swelling, mechanical	Viability, immunohistochemistry		[48]
Müller	Alginate Sulfate, nanocellulose	Bovine articular chondrocytes	Rheology	Viability, morphology, immunohistochemistry, sheer stress of printing		[133]
Müller	Pluronic-diacrylate, pluronic F-127, HAMA, LAP	Bovine articular chondrocytes	Rheology, swelling, FITC release, mechanical, SEM	Viability		[132]
Nedunchezian	Hyaluronic acid-adipic acid dihydrazide-biotin, biotin crosslinked with streptavidin, sodium-alginate, calcium	Human adipose stem cells	Rheology, degradation	Viability, qPCR, histology		[136]
Nguyen	Nanofibrillated cellulose (NFC), hyaluronic acid	Human iPSC and irradiated chondrocytes		Histology, immunohistochemistry, microscopy, qPCR		[138]
Ni	Silk Fibroin/Hydroxypropyl methyl cellulose-methacrylated	Human BMSC	Mechanical, raman spectroscopy	Viability, qPCR		[139]
O’Connell	GelMA, HAMA, LAP	Human adipose stem cells (hADSCs)	Rheology, gel permeation chromatography, mechanical testing, enzymatic crosslinking	Viability, biochemistry, qPCR, microscopy		[142]
Olubamiji	PCL, alginate	ATDC5s		All tests done after in vivo	Subcutaneous in nude mice	[143]
Rathan	Alginate, PCL	Human or pig MSCs	Rheology	Viability, biochemistry, histology, qPCR		[155]
Roh	Oxidized hyaluronate, sodium alginate, glycol chitosan, adipic acid dihydrazide, calcium chloride for crosslinking	ATDC5s		Viability, qPCR		[160]
Ruiz-Cantu	GelMA, I2959, PCL	Sheep articular chondrocytes	Rheology	Viability, genotoxicity, biochemistry, histology, mechanical		[162]
Schipani	GelMA, alginate, PCL	Pig BMSC and articular chondrocytes	Mechanical	Viability, biochemistry, histology, mechanical		[164]
Stichler	Thiol-functionalized hyaluronic acid (HA-SH), P(AGE-co-G), PCL	Human MSCs, Equine MSCs	Mechanical, swelling	Viability, histology, biochemistry		[175]
Sun	Gelatin, fibrinogen, hyaluronic acid, glycerol. PCL. Crosslinked by thrombin solution	Rabbit BMSC		RNA seq, bioinformatics, viability, proliferation, immunofluorescence	Rabbit cartilage knee defect	[179]
Sun	PGLA, PCL	Rabbit BMSC		Viability, mechanical, proliferation, morphology	Subcutaneous in nude mice	[180]
Sun	Gelatin, fibrinogen, hyaluronic acid, glycerol, PCL	GDF5-Rabbit BMSC	Mechanical, degradation	GDF5 release, viability, biomechanical	Rabbit cartilage knee defect	[181]
Trucco	Gelatin, silk-fibroin, alginate	Human MSCs	Rheology, sheer stress	Viability, immunohistochemistry		[184]
Wang	Alginate Sulfate-GelMA, I2959	Pig BMSC	Mechanical, SEM, Swelling, release kinetics, rheology	Viability	Subcutaneous in nude mice	[189]
Yang	Type I collagen, sodium alginate, agarose	Rat articular chondrocytes		Swelling, mechanical, SEM, viability, cytoskeleton, histology, proliferation, biochemistry		[198]
You	Hydroxyapatite, sodium citrate, alginate, PCL	Chicken articular chondrocytes	Rheology, printing fidelity	Viability, proliferation, secretion of cartilage, mineralization	Subcutaneous in nude mice	[201]
Zhang	Decellularized goat ECM, silk fibroin (SF)	Rabbit BMSC	SEM, rheology, spectroscopy, PEG release, degradation, mechanical	Viability, qPCR, biochemistry, histology, growth factor release	Subcutaneous in nude mice	[208]
Zhou	Alginate, gelatin, hyaluronic acid, fibronectin	Rat BMSCs, Chondrogenic progenitor cells	Rheology, mechanical	SEM, viability, confocal, release kinetics, immunofluorescence, qPCR	Rat full thickness cartilage defect	[212]
Zhu	GelMA, PEGDA, 2-Hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone photoinitiator	Human MSCs	Mechanical, swelling	Protein release, proliferation, viability, histology, qPCR		[215]

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