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Fig. 5 | Journal of Experimental Orthopaedics

Fig. 5

From: A decellularized flowable placental connective tissue matrix supports cellular functions of human tenocytes in vitro

Fig. 5

Expression of phenotypic markers in tenocytes cultured on connective tissue matrices over time. The relative expression of tenocyte phenotypic markers (i.e., SCX, TNC, COL1A1, COL3A1, DCN) in tenocytes cultured on A-CTM, I-CTM, and TCP for 2 and 7 days is shown. The relative expression (fold) was compared to the mRNA level in tenocytes on Day 0 (starting cells). Data are plotted as a bar graph (A) and as a heat map (B). Data shown are mean ± SD (n = 4 for cells on connective tissue matrices, n = 3 for cells on TCP). A *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.005. B *p ≤ 0.05, compared with A-CTM; t p ≤ 0.05, compared with TCP; ŧ p ≤ 0.05, compared with day 2. Abbreviations: A-CTM, AmnioFill® connective tissue matrix; COL1A1, type I collagen; COL3A1, type III collagen; DCN, decorin; I-CTM, Interfyl® connective tissue matrix; SCX, scleraxis; TCP, tissue culture polystyrene; TNC, tenascin-C

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