Fig. 2From: Anterior cruciate ligament microfatigue damage detected by collagen autofluorescence in situRepresentative images of SHG and AF images from specimen P1 using benchtop CMM and compiled paired specimen results. A Control ACL SHG, B Fatigue loaded ACL SHG, C Control ACL AF, D Fatigue loaded ACL AF, E The results of all paired knee samples P1 – P3 reported as the intensity of fatigue loaded ACL as a percentage of contralateral control ACL represented by the dashed line at 100%. All fatigue loaded ACLs displayed a reduced SHG signal intensity compared to their contralateral control, whereas the AF signal intensity was enhanced. Red dashed boxes (100 × 100 μm2) indicate regions of interest (ROI) used to quantify SHG and AF intensityBack to article page