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Table 2 Material information and study apparatus

From: Porcine Functional Spine Unit in orthopedic research, a systematic scoping review of the methodology

Study

Level

Breed

Weight

Age

Sample size

Previously frozen

Environmental considerations

Test Equipment

1

Lumbar

na

na

18 months

6

Yes

12 h hydration with phosphate buffer saline solution

Instron 8874

2

Cervical

na

80 kg

6 months

52

Yes

Wrapped in paper wet with saline

Instron 8511

3

Cervical

na

80 kg

6 months

16

Yes

Wrapped in paper wet with saline

Instron 8511

4

Cervical

na

80 kg

6 months

14

na

Heated to body temperature

Instron 8511

5

Lumbar

Domestic

65–73 kg

4 months

16

No, refrigerated

In a plastic bag

MTS Teststar

6

Lumbar

Domestic

 ~ 80 kg

5 months

24

No, refrigerated

In a plastic bag

MTS Teststar

7

Cervical

na

80 kg

6 months

26

Yes

na

Instron 8511

8

Lumbar

na

na

na

32

na

Contained in plastic sleeve filled with saline

Custom made

9

Lumbar

na

na

na

6

na

na

Instron 8874

10

Cervical

na

na

na

16

Yes

Wrapped in saline-soaked cloth

Instron 8872 custom build

11

Lumbar

na

na

 > 16 weeks

6

na

Immersed in an isotonic saline bath cooled to approximately 4 °C

Custom made, 6 DOF

12

Cervical

na

na

na

48

Yes

Moistened with saline every 20 min

Instron 8872

13

Cervical

na

na

na

14

na

Wrapped in saline-soaked gauze

Instron 8872

14

Cervical

na

80 kg

6 months

48

Yes

Wrapped in saline-soaked towel

Instron 8511

15

Lumbar

na

60 kg

na

12

Yes

Sprayed with saline and wrapped in plastic

Custom spine simulator AMTI MC3-A-1000

16

Cervical

na

na

na

96

Yes

na

Instron 8872 custom build

17

Cervical

na

na

na

32

Yes

Wrapped in saline-soaked gauze

Instron 8872 custom build

18

Cervical

na

na

na

30

Yes

na

Instron 8872 Kollmorgen/Danaher Motion AKM23D

19

Cervical

na

na

na

31

Yes

Wrapped in saline-soaked gauze

Instron 8872 Kollmorgen/Danaher Motion AKM23D

20

Thoracic

Domestic

56–61 kg

Immature

14

Yes

Wrapped in moist gauze

Instron 8872

21

Lumbar

Domestic

66 ± 3 kg

Young

12

Yes

na

MTS Teststar

22

Cervical

na

na

na

218

Yes

Wrapped in saline-soaked gauze

Instron 8872

23

Cervical

na

na

na

50

Yes

na

Instron 8872 Kollmorgen/Danaher Motion AKM23D

24

Cervical

na

50–80

na

10

Yes

Wrapped in saline-soaked gauze

Instron 8872

25

Lumbar

Domestic

90–100 kg

8 months

10

Yes

Wrapped in saline-soaked cloth

Instron 8874

26

Cervical

na

Mean 80 kg

Mean 6 months

16

Yes

na

Instron 8511

27

Lumbar

Domestic

65–70 kg

6 months

8

No, refrigerated

Wrapped in saline-soaked gauze

na

28

Lumbar

Domestic

80 kg

Immature

69

Yes

Sprayed with saline

Instron 8872

29

Lumbar

Domestic

mean 78 kg

Mean 7 months

8

Yes

Kept wet by saline-soaked gauze

Instron 8872

30

Cervical

na

 ~ 80 kg

Mean 6 months

22

Yes

Wrapped in saline-soaked cloth

Instron 8511 custom servo motor

31

Lumbar

na

na

na

9

Yes

Wrapped in saline-soaked gauze

Custom built pendulum design

32

Cervical

na

na

na

22

Yes

na

Pressure transducer (model DPG1000DR)

33

Cervical

na

80 kg

6 months

16

Yes

na

Pressure transducer needle (OrthoAR)

34

Cervical

na

na

6–8 months

20

Yes

Wrapped in saline-soaked gauze

Instron 8872

35

Lumbar

na

na

na

1

na

Circulating isotonic saline at 4C

Custom built load device

36

Lumbar

na

na

 > 16 weeks

6

na

Physiological fluid environment

Custom built load device

37

Lumbar

na

na

na

7

na

Room temperature in ambient air

Custom built load device

38

Lumbar

na

na

10 months

8

Yes

Tested in a saline bath at 37 C

Instron 8872

39

Lumbar

na

na

10 months

8

Yes

Tested in a physiologic saline bath (39 °C)

Instron 8872

40

Lumbar

na

na

na

8

na

Wrapped in a saline soaked cloth

Instron 8872

41

Lumbar

na

na

na

6

na

na

na

42

na

na

na

na

1

Yes

na

Custom built load device

43

Cervical

na

na

5–18 months

48

Yes

Encapsulated with plastic-backed saline soaked gauze

Instron 8872 Kollmorgen/Danaher Motion AKM23D

44

Cervical

na

na

na

32

Yes

Temperature-controlled laboratory at 21 °C

Instron 8872 Kollmorgen/Danaher Motion AKM23D

45

Cervical

na

na

na

12

Yes

na

Instron 8872 Kollmorgen/Danaher Motion AKM23D

46

Cervical

na

na

na

24

Yes

Room temperature and surrounded by a water

Instron 8511

47

Cervical

na

na

na

20

Yes

na

Instron 8872

48

Cervical

na

na

na

21

na

Superficial moistening every 20 min

Instron 8872 Kollmorgen/Danaher Motion AKM23D

49

Cervical

na

na

na

30

Yes

Saline soaked cloth wrapped in plastic

Instron 8872 Kollmorgen/Danaher Motion AKM23D

50

Cervical

na

na

na

4

Yes

na

Instron 8511

51

Cervical

na

85 kg

6 months

126

Yes

Hydrated with a saline mist every 15 min

Instron 8872

52

Cervical

na

na

na

14

Yes

Misted with a 0.9% saline solution

Instron 8872

53

Cervical

na

85 kg

6 months

126

Yes

Misted with a saline solution every 15 min

Instron 8872

54

Cervical

na

na

na

18

Yes

Wrapped with saline soaked plastic-backed cloth

Instron 8511 + custom device

55

Cervical

na

na

na

64

Yes

Wrapped in a saline-soaked plastic backed cloth

Instron 8511 + custom device

56

Lumbar

Domestic

55 kg / 195 kg

4 months / 2–3 years

12

Yes

na

MTS Teststar

57

Lumbar

Domestic

75–80 kg

6 months

19

No, refrigerated

Wrapped in saline-soaked gauze

MTS Teststar

58

Cervical

na

80 kg

6 months

30

na

Saline-soaked cloth and plastic wrap

Instron 8511

59

Cervical

na

80 kg

6 months

30

na

Wrapped in cloth soaked in saline along with plastic wrap

Instron 8511

60

Cervical

na

80 kg

6 months

10

na

Saline-soaked cloth and plastic wrap

Instron 8511

61

Lumbar

na

na

6–8 months

5

Yes

Wrapped in a saline soaked towel rehydrated every 20 min

Instron 591 + Instron 8874

62

Cervical

na

80 kg

na

18

Yes

Saline (0.9% NaCl) soaked plastic-backed material and a layer of polythene film

Instron 8511

63

Cervical

na

80 kg

6 months

50

na

Wrapped in a saline soaked cloth and plastic wrap

Instron 8511

64

Cervical

Domestic

80 kg

na

26

Yes

na

Instron 8511

65

Cervical

Domestic

80 kg

6 months

56

Yes

na

Instron 8511

66

Cervical

na

80 kg

na

na

Yes

na

Instron 8511

67

Lumbar

na

na

na

1

Yes

Sprayed and wrapped in paper towel soaked with 0.9% saline solution, triple sealed in plastic bag

Dynamic six-axis spine simulator, dSPACE Ltd

68

Lumbar

Organically farmed pig

60 kg

8–12 months

1

No

wrapped in plastic film at room temperature (20 C)

Zwick 25–200

69

Cervical

na

na

na

48

Yes

3% weight/volume saline soaked tissue

Instron 8872 Kollmorgen/Danaher Motion AKM23D

70

Cervical

na

60 kg

na

28

Yes

na

pressure transducer, model DPG1000DR; 2000 PSIG transducer