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Table 1 Eight weeks of culture in CellCrown®. Perforated PET membrane in all groups. Some groups weren’t pretreated with collagenase while the others were predigested with both low and high enzyme concentrations. Some groups didn’t contain any scaffold, some groups contained fibrin, some groups EZ-derm®, while other groups contained chitosan-based scaffold. Upper compartment with PET membrane was fixated, emerged in paraffin and histology sections were made. Hematoxylin, eosin and trichrome staining was done. Immunohistochemistry analysis for four markers was performed: procollagen type I and II, SOX-9 and S-100

From: The enhancement of cartilage regeneration by use of a chitosan-based scaffold in a 3D model of microfracture in vitro: a pilot evaluation

Well

Plate

Bone

Cartilage

Collagenase (conc.)

Fibrin

EZ-derm

Chitosan

1

A

 + 

 + 

-

-

-

 + 

2

A

 + 

 + 

-

-

 + 

-

3

A

 + 

 + 

-

-

 + 

-

4

A

 + 

 + 

-

 + 

 + 

-

5

A

 + 

 + 

-

-

 + 

-

6

A

 + 

 + 

-

 + 

 + 

-

7

A

 + 

 + 

-

-

 + 

-

8

B

 + 

 + 

Low

-

-

 + 

9

B

 + 

 + 

Low

-

 + 

-

10

B

 + 

 + 

Low

 + 

 + 

-

11

B

 + 

 + 

Low

-

 + 

-

12

B

 + 

 + 

Low

 + 

 + 

-

13

B

 + 

 + 

Low

-

 + 

-

14

B

 + 

 + 

High

-

-

 + 

15

B

 + 

 + 

High

-

 + 

-

16

B

 + 

 + 

High

 + 

 + 

-

17

B

 + 

 + 

High

-

 + 

-

18

B

 + 

 + 

High

 + 

 + 

-

19

B

 + 

 + 

High

-

 + 

-

20

B

-

 + 

High

-

 + 

-

21

A

 + 

-

-

-

 + 

-