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Fig. 2 | Journal of Experimental Orthopaedics

Fig. 2

From: Identifying potential patient-specific predictors for anterior cruciate ligament reconstruction outcome – a diagnostic in vitro tissue remodeling platform

Fig. 2

Steps to obtain top-view micro-tissue surface area. (A) Based on absolute intensity values: the original image (A1) was binarized (A2), the largest connected component was selected, and any remaining holes were filled (A3). The object size (in pixels) was counted and converted to the absolute surface area. In case strategy A failed, strategy B was applied. (B) Based on micro-tissue: on the original image (B1) edge detection was performed (B2). Image 2 was binarized (B3), and subtracted from the original image (B4). The resulting image was binarized (B5), the largest connected component was selected, and any remaining holes were filled (B6)

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