Fig. 5

An evaluation of engineered cartilage treated with TD198946 and BMP-2 in vivo. a Macroscopic views of sacrificed mice and implanted engineered cartilages. (n = 5) 3-D μ-CT scan image of implanted engineered cartilages treated with combination of 10 nM TD198946 and 100 ng/mL BMP-2 below the macro view. (n = 2) b Histological images for Safranin-O, IHC for type I, II and X collagen. Scale bars = 2 mm (Low magnification: upper panels) and 100 μm (High magnification: below Low magnification). Upper images: high magnification views of central zone. Lower images: high magnification views of surface zone. High magnification panels are magnified images of the specific zone represented by the square on the low magnification view. c Quantitative area measurement of type I, II and X collagen. (n = 5) Mean ± SD, *: p < 0.05. Abbreviations: μ-CT, micro-computed tomography; IHC, Immunohistochemistry