Fig. 6From: Optimization of leukocyte-poor platelet-rich plasma preparation: a validation study of leukocyte-poor platelet-rich plasma obtained using different preparer, storage, and activation methodsScheme of the the centrifuged blood with PRGF-Endoret protocol. A line was drawn 5 mm above the buffy-coat layer in the separated blood, and the layer above this line was divided into two. The upper portion was designated as PPP, and the lower portion was designated as PRP. a The target layer (shaded area) of the PRP system, containing LP-PRP. b When aspirating from above the target layer (shaded area), the platelet concentration obtained was relatively low, without contaminating leukocytes, representing purified PRP. c Aspirating from layers below the target layer (shaded area), platelet concentration increased, though leukocytes were retained, which was leukocyte-rich PRPBack to article page