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Fig. 1 | Journal of Experimental Orthopaedics

Fig. 1

From: Absence of cytotoxic and inflammatory effects following in vitro exposure of chondrogenically-differentiated human mesenchymal stem cells to adenosine, lidocaine and Mg2+ solution

Fig. 1

Chondrogenic differentiation of human MSC. Cellular morphology changes were evident in human MSC (a) prior to, and (b) following culture in chondrogenic differentiation media for 7 days, with the transformation of spindle-shaped MSC to rounded, polygonal chondrocytes (magnification, 10x). Chondrogenic differentiation was confirmed histologically, with chondrocyte aggregation and early structural organisation of a collagenous matrix evident. Representative formalin-fixed, paraffin-embedded sections of chondro-MSC were stained with (c) toluidine blue to demonstrate sulphated glycosaminoglycans deposition (white arrow) or (d) Picro-Mallory trichrome stain highlighting deposition of collagen fibres (black arrowhead) within the extracellular matrix (magnification, 20x)

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