Fig. 1

Development of the tissue-engineered construct (TEC). a Macroscopic view of the TEC that was integrated to one spherical body. b Hematoxylin and eosin staining of TEC. c Immunohistochemical analysis of the TEC stained with type I collagen (Col I), type II collagen (Col II), type III collagen (Col III), fibronectin, vitronectin, and negative IgG (control). Red are nuclei and green is target antibody. Adhesion molecules such as fibronectin and vitronectin are diffusely distributed within the TEC. Bar = 100 μm. Quoted and modified from Ando et al., Biomaterials 2007 and Shimomura et al. Cartilage 2015