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Table 5 Histomorphometric analyses in the rAAV-transduced human bone marrow aspirates (day 7)

From: Impact of mechanical stimulation on the chondrogenic processes in human bone marrow aspirates modified to overexpress sox9 via rAAV vectors

Assay Static culture Dynamic culture
no vector luc sox9 no vector luc sox9
Safranin O
staining
0.3
(0.2)
0.3
(0.1)
2.0
(0.2)a,b
0.2
(0.1)
0.3
(0.1)
2.6
(0.3)a,b,c
Type-II collagen
immunostaining
0.2
(0.1)
0.3
(0.1)
2.8
(0.3)a,b
0.4
(0.1)
0.4
(0.2)
3.3
(0.2)a,b,c
Alizarin red
staining
0.2
(0.1)
0.2
(0.2)
0.1
(0.1)
0.3
(0.2)
01.
(0.1)
0.2
(0.1)
Type-X collagen
immunostaining
0.1
(0.1)
0.1
(0.1)
0.2
(0.1)
0.2
(0.1)
0.2
(0.1)
0.1
(0.1)
  1. Values are given as mean (SD) with n = 4 independent samples tested in triplicate per vector treatment and culture condition in three independent experiments. Safranin O and alizarin red staining and type-II and -X collagen immunostaining were scored for uniformity and intensity according to a modified Bern Score grading system (Rey-Rico et al. 2015b) as: 0 (no staining), 1 (heterogeneous and/or weak staining), 2 (homogeneous and/or moderate staining), 3 (homogeneous and/or intense staining), and 4 (very intense staining). Statistically significant relative to ano vector treatment, brAAV-luc application, and cstatic culture