Assay | Static culture | Dynamic culture |
---|
no vector |
luc
|
sox9
| no vector |
luc
|
sox9
|
---|
Safranin O staining | 0.3 (0.2) | 0.3 (0.1) | 2.0 (0.2)a,b
| 0.2 (0.1) | 0.3 (0.1) | 2.6 (0.3)a,b,c
|
Type-II collagen immunostaining | 0.2 (0.1) | 0.3 (0.1) | 2.8 (0.3)a,b
| 0.4 (0.1) | 0.4 (0.2) | 3.3 (0.2)a,b,c
|
Alizarin red staining | 0.2 (0.1) | 0.2 (0.2) | 0.1 (0.1) | 0.3 (0.2) | 01. (0.1) | 0.2 (0.1) |
Type-X collagen immunostaining | 0.1 (0.1) | 0.1 (0.1) | 0.2 (0.1) | 0.2 (0.1) | 0.2 (0.1) | 0.1 (0.1) |
- Values are given as mean (SD) with n = 4 independent samples tested in triplicate per vector treatment and culture condition in three independent experiments. Safranin O and alizarin red staining and type-II and -X collagen immunostaining were scored for uniformity and intensity according to a modified Bern Score grading system (Rey-Rico et al. 2015b) as: 0 (no staining), 1 (heterogeneous and/or weak staining), 2 (homogeneous and/or moderate staining), 3 (homogeneous and/or intense staining), and 4 (very intense staining). Statistically significant relative to ano vector treatment, brAAV-luc application, and cstatic culture