Impact of mechanical stimulation on the chondrogenic processes in human bone marrow aspirates modified to overexpress sox9 via rAAV vectors

Journal of Experimental Orthopaedics

Table 5 Histomorphometric analyses in the rAAV-transduced human bone marrow aspirates (day 7)

Assay	Static culture			Dynamic culture
Assay	no vector	luc	sox9	no vector	luc	sox9
Safranin O staining	0.3 (0.2)	0.3 (0.1)	2.0 (0.2)^a,b	0.2 (0.1)	0.3 (0.1)	2.6 (0.3)^a,b,c
Type-II collagen immunostaining	0.2 (0.1)	0.3 (0.1)	2.8 (0.3)^a,b	0.4 (0.1)	0.4 (0.2)	3.3 (0.2)^a,b,c
Alizarin red staining	0.2 (0.1)	0.2 (0.2)	0.1 (0.1)	0.3 (0.2)	01. (0.1)	0.2 (0.1)
Type-X collagen immunostaining	0.1 (0.1)	0.1 (0.1)	0.2 (0.1)	0.2 (0.1)	0.2 (0.1)	0.1 (0.1)

Values are given as mean (SD) with n = 4 independent samples tested in triplicate per vector treatment and culture condition in three independent experiments. Safranin O and alizarin red staining and type-II and -X collagen immunostaining were scored for uniformity and intensity according to a modified Bern Score grading system (Rey-Rico et al. 2015b) as: 0 (no staining), 1 (heterogeneous and/or weak staining), 2 (homogeneous and/or moderate staining), 3 (homogeneous and/or intense staining), and 4 (very intense staining). Statistically significant relative to ^ano vector treatment, ^brAAV-luc application, and ^cstatic culture