Assay | Static culture | Dynamic culture | ||||
---|---|---|---|---|---|---|
no vector | luc | sox9 | no vector | luc | sox9 | |
Cell densities (cells/mm2) | 4389 (88) | 4987 (91) | 5084 (72) | 6796 (98)c | 7046 (103)c | 7892 (76)c |
WST-1 (OD450 nm) | 0.298 (0.024) | 0.304 (0.032) | 0.328 (0.037) | 0.787 (0.095)c | 0.881 (0.088)c | 0.904 (0.063)c |
DNA (μg/mg total proteins) | 0.29 (0.08) | 0.30 (0.11) | 0.34 (0.09) | 0.35 (0.12)c | 0.32 (0.14)c | 0.38 (0.09)c |
Proteoglycans (mg/mg total proteins) | 0.009 (0.002) | 0.010 (0.002) | 0.012 (0.004)a,b | 0.011 (0.002)c | 0.012 (0.003)c | 0.017 (0.003)a,b,c |
Proteoglycans (mg/μg DNA) | 0.031 (0.003) | 0.033 (0.003) | 0.037 (0.002)a,b | 0.034 (0.002)c | 0.037 (0.002)c | 0.045 (0.004)a,b,c |
Type-II collagen (μg/mg total proteins) | 0.016 (0.002) | 0.018 (0.002) | 0.023 (0.001)a,b | 0.020 (0.001)c | 0.021 (0.002)c | 0.039 (0.002)a,b,c |
Type-II collagen (μg/μg DNA) | 0.055 (0.002) | 0.058 (0.003) | 0.067 (0.003)a,b | 0.062 (0.002)c | 0.065 (0.002)c | 0.102 (0.003)a,b,c |
Type-X collagen (μg/mg total proteins) | 0.021 (0.003) | 0.022 (0.004) | 0.014 (0.003)a,b | 0.019 (0.002) | 0.020 (0.003) | 0.010 (0.002)a,b |
Type-X collagen (μg/μg DNA) | 0.072 (0.002) | 0.073 (0.003) | 0.041 (0.003)a,b | 0.069 (0.003) | 0.071 (0.002) | 0.026 (0.002)a,b |