Skip to main content

Table 2 Biochemical assays in the rAAV-transduced human bone marrow aspirates (day 28)

From: Impact of mechanical stimulation on the chondrogenic processes in human bone marrow aspirates modified to overexpress sox9 via rAAV vectors

Assay

Static culture

Dynamic culture

no vector

luc

sox9

no vector

luc

sox9

Cell densities

(cells/mm2)

4389

(88)

4987

(91)

5084

(72)

6796

(98)c

7046

(103)c

7892

(76)c

WST-1

(OD450 nm)

0.298

(0.024)

0.304

(0.032)

0.328

(0.037)

0.787

(0.095)c

0.881

(0.088)c

0.904

(0.063)c

DNA

(μg/mg total proteins)

0.29

(0.08)

0.30

(0.11)

0.34

(0.09)

0.35

(0.12)c

0.32

(0.14)c

0.38

(0.09)c

Proteoglycans

(mg/mg total proteins)

0.009

(0.002)

0.010

(0.002)

0.012

(0.004)a,b

0.011

(0.002)c

0.012

(0.003)c

0.017

(0.003)a,b,c

Proteoglycans

(mg/μg DNA)

0.031

(0.003)

0.033

(0.003)

0.037

(0.002)a,b

0.034

(0.002)c

0.037

(0.002)c

0.045

(0.004)a,b,c

Type-II collagen

(μg/mg total proteins)

0.016

(0.002)

0.018

(0.002)

0.023

(0.001)a,b

0.020

(0.001)c

0.021

(0.002)c

0.039

(0.002)a,b,c

Type-II collagen

(μg/μg DNA)

0.055

(0.002)

0.058

(0.003)

0.067

(0.003)a,b

0.062

(0.002)c

0.065

(0.002)c

0.102

(0.003)a,b,c

Type-X collagen

(μg/mg total proteins)

0.021

(0.003)

0.022

(0.004)

0.014

(0.003)a,b

0.019

(0.002)

0.020

(0.003)

0.010

(0.002)a,b

Type-X collagen

(μg/μg DNA)

0.072

(0.002)

0.073

(0.003)

0.041

(0.003)a,b

0.069

(0.003)

0.071

(0.002)

0.026

(0.002)a,b

  1. Values are given as mean (SD) with n = 4 independent samples tested in triplicate per vector treatment and culture condition in three independent experiments. Statistically significant relative to ano vector treatment, brAAV-luc application, and cstatic