Assay | Static culture | Dynamic culture | ||||
---|---|---|---|---|---|---|
no vector | luc | sox9 | no vector | luc | sox9 | |
Cell densities (cells/mm2) | 4556 (94) | 5453 (87) | 5641 (95) | 7044 (104)c | 9101 (112)c | 9157 (108)c |
WST-1 (OD450 nm) | 0.320 (0.031) | 0.351 (0.046) | 0.402 (0.048) | 1.037 (0.118)c | 1.002 (0.107)c | 1.056 (0.122)c |
DNA (μg/mg total proteins) | 0.31 (0.12) | 0.32 (0.17) | 0.35 (0.17) | 0.36 (0.14)c | 0.37 (0.17)c | 0.42 (0.16)c |
Proteoglycans (mg/mg total proteins) | 0.006 (0.002) | 0.007 (0.003) | 0.009 (0.005)a,b | 0.009 (0.002)c | 0.010 (0.003)c | 0.016 (0.004)a,b,c |
Proteoglycans (mg/μg DNA) | 0.019 (0.001) | 0.022 (0.002) | 0.026 (0.003)a,b | 0.025 (0.001)c | 0.027 (0.002)c | 0.038 (0.002)a,b,c |
Type-II collagen (μg/mg total proteins) | 0.018 (0.002) | 0.019 (0.001) | 0.024 (0.001)a,b | 0.021 (0.001)c | 0.022 (0.002)c | 0.040 (0.003)a,b,c |
Type-II collagen (μg/μg DNA) | 0.058 (0.003) | 0.059 (0.002) | 0.069 (0.003)a,b | 0.065 (0.002)c | 0.068 (0.003)c | 0.095 (0.004)a,b,c |
Type-X collagen (μg/mg total proteins) | 0.022 (0.004) | 0.024 (0.007) | 0.012 (0.006)a,b | 0.020 (0.003) | 0.021 (0.005) | 0.011 (0.002)a,b |
Type-X collagen (μg/μg DNA) | 0.071 (0.003) | 0.075 (0.004) | 0.034 (0.004)a,b | 0.068 (0.002) | 0.070 (0.004) | 0.026 (0.002)a,b |