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Table 1 Biochemical assays in the rAAV-transduced human bone marrow aspirates (day 21)

From: Impact of mechanical stimulation on the chondrogenic processes in human bone marrow aspirates modified to overexpress sox9 via rAAV vectors

Assay Static culture Dynamic culture
no vector luc sox9 no vector luc sox9
Cell densities
(cells/mm2)
4556
(94)
5453
(87)
5641
(95)
7044
(104)c
9101
(112)c
9157
(108)c
WST-1
(OD450 nm)
0.320
(0.031)
0.351
(0.046)
0.402
(0.048)
1.037
(0.118)c
1.002
(0.107)c
1.056
(0.122)c
DNA
(μg/mg total proteins)
0.31
(0.12)
0.32
(0.17)
0.35
(0.17)
0.36
(0.14)c
0.37
(0.17)c
0.42
(0.16)c
Proteoglycans
(mg/mg total proteins)
0.006
(0.002)
0.007
(0.003)
0.009
(0.005)a,b
0.009
(0.002)c
0.010
(0.003)c
0.016
(0.004)a,b,c
Proteoglycans
(mg/μg DNA)
0.019
(0.001)
0.022
(0.002)
0.026
(0.003)a,b
0.025
(0.001)c
0.027
(0.002)c
0.038
(0.002)a,b,c
Type-II collagen
(μg/mg total proteins)
0.018
(0.002)
0.019
(0.001)
0.024
(0.001)a,b
0.021
(0.001)c
0.022
(0.002)c
0.040
(0.003)a,b,c
Type-II collagen
(μg/μg DNA)
0.058
(0.003)
0.059
(0.002)
0.069
(0.003)a,b
0.065
(0.002)c
0.068
(0.003)c
0.095
(0.004)a,b,c
Type-X collagen
(μg/mg total proteins)
0.022
(0.004)
0.024
(0.007)
0.012
(0.006)a,b
0.020
(0.003)
0.021
(0.005)
0.011
(0.002)a,b
Type-X collagen
(μg/μg DNA)
0.071
(0.003)
0.075
(0.004)
0.034
(0.004)a,b
0.068
(0.002)
0.070
(0.004)
0.026
(0.002)a,b
  1. Values are given as mean (SD) with n = 4 independent samples tested in triplicate per vector treatment and culture condition in three independent experiments. Statistically significant relative to ano vector treatment, brAAV-luc application, and cstatic culture