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Journal of Experimental Orthopaedics
Fig. 5 | Journal of Experimental Orthopaedics

Fig. 5

From: Effects of combined rAAV-mediated TGF-β and sox9 gene transfer and overexpression on the metabolic and chondrogenic activities in human bone marrow aspirates

Fig. 5

Expression analyses via real-time RT-PCR in human bone marrow aspirates co-transduced with rAAV-hTGF-β/rAAV-FLAG-hsox9. Aspirates were treated and maintained in culture as described in Figs. 1, 2, 3 and 4. Total cellular RNA extraction and cDNA synthesis with subsequent analysis of gene expression via real-time RT-PCR amplification was performed after 21 days as described in the Materials and Methods to generate Ct values for each target. The transcription factor SOX9, aggrecan (ACAN), type-II collagen (COL2A1), type-I collagen (COL1A1), and type-X collagen (COL10A1) were analyzed, with GAPDH serving as a housekeeping gene and internal control for normalization (primers are listed in the Materials and Methods). Fold inductions were measured for each target by using the 2-ΔΔCt method relative to the control groups (rAAV-lacZ, no vector condition). *,**Statistically significant compared with control groups (rAAV-lacZ, no vector condition) (*p ≤ 0.050, **p ≤ 0.010)

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