Table 5 Results on changes in cellular and/or signaling response to BMMSC cultivated constructs under hydrostatic compression (Fig. 2). Sox9 is the gene that regulates chondrogenic differentiation; h is hours; h/day is hours per day; − is decrease or inhibition; and + is increase; ++ is highest increase; = is no change or status quo
From: What quantitative mechanical loading stimulates in vitro cultivation best?
Study | Cell source, cultivated construct, additive(s) | Magnitude (MPa) | Freq (Hz) | Loading (h/day) | Loading (# days) | Loading (total h) | Culture composition (change -, =, +) | mRNA response (change -, =, +) |
|---|---|---|---|---|---|---|---|---|
Mesh | ||||||||
Wagner 2008 (Wagner et al. 2008) | Human BMMSC, Mesh Collagen Type 1 50 mg/mL bovine serum albumin; 50 μg/mL L-ascorbic acid; 10−9 M dexamethasone | 1 | 1 | 4 | 10 | 40 | + proteoglycan | + Sox9 |
+ aggrecan | ||||||||
+ collagen II | ||||||||
Luo 2007 (Luo and Seedhom 2007) | Ovine BMMSC, Mesh non-woven filamentous plasma-treated polyester 10 % Fetal bovine serum; 50 μg/mL ascorbic acid; 10−7 M dexamethasone | 0.1 | 0.25 | 0.5 | 7 | 3.5 | + GAG |  |
= collagen | ||||||||
0.5 | 10 | 5 | ++ GAG | Â | ||||
+ collagen | ||||||||
Gel | ||||||||
Miyanishi 2006a (Miyanishi et al. 2006a) | Human BMMSC, Aggregate pellet 1.25 mg/mL bovine serum albumin; 50 μg/mL ascorbic acid; 10−7 M dexamethasone | 0.1 | 1 | 4 | 14 | 56 | = sGAG | + Sox9 |
+ aggrecan | ||||||||
= collagen II | ||||||||
1 | + sGAG | ++ Sox9 | ||||||
+ aggrecan | ||||||||
= collagen II | ||||||||
10 | ++ sGAG | ++ Sox9 | ||||||
++ aggrecan | ||||||||
+ collagen II | ||||||||
Miyanishi 2006b (Miyanishi et al. 2006b) | Human BMMSC, Aggregate pellet 1.25 mg/mL bovine serum albumin; 50 cpg/mL ascorbic acid; 10−7 M dexamethasone | 10 | 1 | 4 | 14 | 56 |  | + Sox9 |
+ aggrecan | ||||||||
+ collagen II | ||||||||
Angele 2003 (Angele et al. 2003) | Human BMMSC, Aggregate pellet 10Â % Fetal bovine serum | 5.03 | 1 | 4 | 1 | 4 | = proteoglycan | Â |
= collagen | ||||||||
4 | 7 | 28 | + proteoglycan | Â | ||||
+ collagen | ||||||||
Finger 2007 (Finger et al. 2007) | Human BMMSC, Agarose gel Type VII Growth medium Cambrex | 7.5 | 1 | 4 | 14 | 56 | Â | = Sox9 |