Polymerase Chain Reaction molecular diagnostic technology for monitoring chronic osteomyelitis

Journal of Experimental Orthopaedics

Table 1 Rabbit Osteomyelitis (Group 1): comparison of infection detection methods

		Infection detection
Animal no.	Inoculum (CFU)	Microbiological culture	Radiography	PCR test*
1	1 × 10⁶	+	+	+
2	1 × 10⁶	+	+	+
3	1 × 10⁶	+	+	+
4	1 × 10⁶	+	+	+
5	1 × 10⁴	+	+	+
6	1 × 10⁴	-	+	+
7	1 × 10⁴	+	+	+**
8	Sclerosing agent and saline only	-	-	-
9	Unoperated control	-	-	-

*PCR done using 16S rRNA gene primers.
**Positive PCR result obtained after two rounds of extraction of sample with Mixed Bed Ion Exchange Resin (see text for details).