Effects of rAAV-mediated FGF-2 gene transfer and overexpression upon the chondrogenic differentiation processes in human bone marrow aspirates

Journal of Experimental Orthopaedics

Table 1 Primers used for real-time RT-PCR

Gene	Primer sequences (5’-3’)
ACAN^a	GAGATGGAGGGTGAGGTC ACGCTGCCTCGGGCTTC
COL2A1^a	GGACTTTTCTCCCCTCTCT GACCCGAAGGTCTTACAGGA
SOX9^a	ACACACAGCTCACTCGACCTTG GGGAATTCTGGTTGGTCCTCT
COL1A1^b	ACGTCCTGGTGAAGTTGGTC ACCAGGGAAGCCTCTCTCTC
COL10A1^c	CCCTCTTGTTAGTGCCAACC AGATTCCAGTCCTTGGGTCA
MMP13^c	AATTTTCACTTTTGGCAATGA CAAATAATTTATGAAAAAGGGATGC
ALP^b	TGGAGCTTCAGAAGCTCAACACCA ATCTCGTTGTCTGAGTACCAGTCC
RUNX2^b	GCAGTTCCCAAGCATTTCAT CACTCTGGCTTTGGGAAGAG
GAPDH^d	GAAGGTGAAGGTCGGAGTC GAAGATGGTGATGGGATTTC

SOX9 SRY (sex determining region Y)-box 9, COL2A1 type-II collagen α1, ACAN aggrecan, COL1A1 type-I collagen α1, COL10A1 type-X collagen α1, MMP13 matrix metallo-proteinase 13, ALP alkaline phosphatase, RUNX2 runt-related transcription factor 2, GAPDH glyceraldehyde-3-phosphate dehydrogenase. ^aChondrogenic markers; ^bOsteogenic markers; ^cHypertrophic and terminal differentiation markers; ^dHousekeeping gene (control)